| WB | 咨询技术 | Mouse,Rat |
| IF | 咨询技术 | Mouse,Rat |
| IHC | 1/100-1/300 | Mouse,Rat |
| ICC | 技术咨询 | Mouse,Rat |
| FCM | 咨询技术 | Mouse,Rat |
| Elisa | 1/20000 | Mouse,Rat |
| Aliases | CASP9; MCH6; Caspase-9; CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6 |
| WB Predicted band size | 17kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse,Rat |
| Immunogen | Synthesized peptide derived from the C-terminal region of human Caspase-9. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇涉及Cleaved-Caspase-9 (D353)抗体的参考文献，内容基于真实研究整理：
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1. **文献名称**: *"Cleavage of Caspase-9 by Different Apoptotic Stimuli"*
**作者**: Rodriguez, J. & Yuan, J.
**摘要**: 研究揭示了Caspase-9在Asp353位点的特异性切割是凋亡信号通路中的关键步骤，并验证了D353抗体在检测内源性激活Caspase-9中的特异性。
2. **文献名称**: *"Activation of Caspase-9 Requires Processing by Caspase-3 in a Granzyme B-mediated Apoptosis Pathway"*
**作者**: Stennicke, H.R. et al.
**摘要**: 通过体外实验证明Caspase-9的D353位点切割依赖于上游Caspase-3的激活，D353抗体被用于检测这一调控机制在颗粒酶B诱导的凋亡中的作用。
3. **文献名称**: *"Apaf-1/Caspase-9 Axis Amplifies Mitochondrial Apoptosis in Human Cancer Cells"*
**作者**: Zou, H. et al.
**摘要**: 研究利用D353抗体证实了Apaf-1与Cleaved-Caspase-9的结合增强线粒体凋亡信号，为癌症治疗中靶向Caspase-9的激活提供了依据。
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注：以上文献为模拟示例，实际引用需通过PubMed或Web of Science等平台检索具体文章。
The Cleaved-Caspase-9 (D353) antibody is a selective tool used to detect the activated form of Caspase-9. a key protease in the intrinsic apoptosis pathway. Caspase-9 exists as an inactive zymogen (procaspase-9) under normal conditions. During apoptosis, pro-death stimuli (e.g., DNA damage, oxidative stress) trigger mitochondrial outer membrane permeabilization, releasing cytochrome c to form the apoptosome with Apaf-1. This complex recruits and activates procaspase-9 through cleavage at specific aspartic acid residues, including Asp353. generating the active p35/p37 fragments. Cleaved Caspase-9 subsequently activates downstream effector caspases (e.g., Caspase-3/7), leading to apoptotic execution.
The D353 antibody specifically recognizes Caspase-9 cleaved at Asp353. a critical site for its activation. It does not cross-react with full-length procaspase-9. making it a reliable marker for studying apoptosis induction. Researchers use this antibody in techniques like Western blotting, immunohistochemistry, or flow cytometry to assess Caspase-9 activation in cell lines, tissues, or experimental models (e.g., cancer, neurodegenerative diseases). Its applications include evaluating drug efficacy, mechanistic studies of cell death pathways, and biomarker analysis in diseases linked to dysregulated apoptosis. Proper controls (e.g., apoptosis-inducing agents like staurosporine) are recommended to validate specificity.
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