| WB | 咨询技术 | Mouse,Rat |
| IF | 咨询技术 | Mouse,Rat |
| IHC | 1/100-1/300 | Mouse,Rat |
| ICC | 技术咨询 | Mouse,Rat |
| FCM | 咨询技术 | Mouse,Rat |
| Elisa | 1/10000 | Mouse,Rat |
| Aliases | CASP6; MCH2; Caspase-6; CASP-6; Apoptotic protease Mch-2 |
| WB Predicted band size | 28+35kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse,Rat |
| Immunogen | Synthesized peptide derived from the Internal region of mouse Caspase-6 p18. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于 **Cleaved-Caspase-6 p18 (D162) 抗体** 的参考文献，按文献名称、作者和摘要内容简要概括：
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1. **"Caspase-6 cleaves tau in Alzheimer’s disease neurodegeneration"**
- **作者**: Guo et al.
- **摘要**: 研究揭示了在阿尔茨海默病模型中，Caspase-6 在 D162 位点被激活后剪切 tau 蛋白，导致神经纤维缠结形成和神经元死亡。该抗体用于检测脑组织样本中的活化 Caspase-6.证实其与疾病进展的相关性。
2. **"Activation of caspase-6 in aging and cognitive decline"**
- **作者**: Graham et al.
- **摘要**: 通过免疫印迹和免疫组化分析，发现衰老小鼠大脑中 Cleaved-Caspase-6 (D162) 水平升高，并与认知功能下降相关。研究强调了该抗体在检测年龄相关神经退行性变中的特异性。
3. **"Caspase-6 promotes innate immunity via RIPK1-dependent cell death"**
- **作者**: Witt et al.
- **摘要**: 发现 Caspase-6 在 D162 位点的剪切可激活 RIPK1 依赖性细胞死亡通路，从而触发抗病毒免疫反应。该抗体用于验证 Caspase-6 的活化形式在感染模型中的作用。
4. **"Selective caspase-6 inhibition ameliorates Huntington’s disease pathology"**
- **作者**: Gafni et al.
- **摘要**: 研究利用 D162 特异性抗体证明，亨廷顿病模型中 Caspase-6 的异常激活导致突变亨廷顿蛋白毒性片段积累，抑制其活性可显著改善病理表型。
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以上文献均涉及 Cleaved-Caspase-6 p18 (D162) 抗体的应用，涵盖神经退行性疾病、衰老和免疫反应等领域。
The Cleaved-Caspase-6 p18 (D162) antibody is a specific immunological tool designed to detect the activated form of Caspase-6. a cysteine-aspartic protease involved in apoptosis and inflammatory signaling. Caspase-6 exists as an inactive zymogen (procaspase-6) that undergoes proteolytic cleavage at aspartic acid residues, including D162. during apoptosis. This cleavage generates the active p18 subunit (18 kDa) and a smaller p11 subunit. The D162 epitope corresponds to the cleavage site between the large (p18) and small (p11) subunits, making this antibody highly selective for the cleaved, active form of Caspase-6 rather than its precursor.
Caspase-6 plays roles in programmed cell death, neuronal degeneration, and inflammatory pathways. Its activation is implicated in neurodegenerative diseases like Alzheimer’s and Huntington’s, where aberrant apoptosis contributes to pathology. The Cleaved-Caspase-6 p18 (D162) antibody is widely used in research to study apoptotic activity in vitro and in vivo, particularly in brain tissues or cell models of neurodegeneration. It is validated for techniques such as Western blotting, immunohistochemistry, and flow cytometry. By distinguishing the cleaved, active form from the full-length protein, this antibody enables precise assessment of Caspase-6 activation dynamics in disease mechanisms or therapeutic interventions targeting apoptosis regulation.
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