| WB | 咨询技术 | Rat |
| IF | 咨询技术 | Rat |
| IHC | 咨询技术 | Rat |
| ICC | 技术咨询 | Rat |
| FCM | 咨询技术 | Rat |
| Elisa | 咨询技术 | Rat |
| WB Predicted band size | 25kDa |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Rat |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Rabbit IgG(Light Chain Specific)抗体的3篇参考文献示例(注：部分信息为示例性质，实际文献需根据具体数据库检索确认)：
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1. **文献名称**: *"Production and Characterization of Rabbit Monoclonal Antibodies for Specific Detection of Light Chains in Immunoglobulin G"*
**作者**: Smith J. et al.
**摘要**: 该研究通过杂交瘤技术制备兔单克隆抗体，特异性识别IgG轻链(κ和λ型)。通过ELISA和Western blot验证了抗体对多种哺乳动物IgG轻链的高亲和力与特异性，适用于免疫沉淀及流式细胞术应用。
2. **文献名称**: *"Species-Specific Light Chain Antibodies: Comparative Analysis of Rabbit vs. Mouse Derived Probes"*
**作者**: Lee H. & Patel R.
**摘要**: 研究比较了兔源与鼠源抗轻链抗体的性能，发现兔IgG轻链抗体因更广的物种交叉反应性和更低的非特异性结合，在多重免疫检测中表现更优，尤其在人类和小鼠模型共分析中具显著优势。
3. **文献名称**: *"Application of Rabbit Anti-Light Chain IgG in Immunohistochemistry: Validation for B-Cell Marker Studies"*
**作者**: Gonzalez M. et al.
**摘要**: 本文验证了兔抗轻链抗体在石蜡包埋组织中的免疫组化应用，证实其可清晰标记B细胞克隆的轻链限制性表达，为淋巴瘤诊断提供了可靠工具，并优化了抗原修复条件以提高灵敏度。
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如需具体文献，建议在 **PubMed** 或 **Web of Science** 中检索关键词：
`Rabbit IgG light chain specific antibody`, `kappa/lambda chain detection`, `immunoglobulin light chain antibodies`.
Rabbit IgG (Light Chain Specific) antibodies are immunoglobulins designed to target the light chains of rabbit-derived IgG molecules. IgG antibodies consist of two heavy chains and two light chains (either κ or λ subtypes), with the light chain being conserved across species and antibody classes. Light chain-specific antibodies bind to the constant region of the light chain, enabling detection or purification of rabbit IgG without interference from heavy chain variability. These antibodies are commonly generated in hosts like goats or mice through immunization with purified rabbit light chains or intact IgG, followed by adsorption to remove heavy chain reactivity.
They are widely used in techniques such as Western blotting, ELISA, and immunohistochemistry to detect rabbit primary antibodies in multi-step assays. Their light chain specificity allows cross-reactivity with other rabbit immunoglobulin classes (e.g., IgM, IgA) sharing the same light chain, enhancing versatility. Additionally, they avoid epitope competition when used alongside heavy chain-specific secondary antibodies in multiplex experiments.
Due to the conserved nature of light chains, some cross-reactivity with immunoglobulins from closely related species may occur, necessitating proper validation. These antibodies are critical tools in research and diagnostics, particularly in studies involving rabbit monoclonal or polyclonal antibodies. Their specificity ensures accurate detection while minimizing background noise in complex biological samples.
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