| WB | 咨询技术 | Rat |
| IF | 咨询技术 | Rat |
| IHC | 咨询技术 | Rat |
| ICC | 技术咨询 | Rat |
| FCM | 咨询技术 | Rat |
| Elisa | 咨询技术 | Rat |
| WB Predicted band size | 55kDa |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Rat |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇关于Rabbit IgG (Fc specific, Heavy Chain)抗体的参考文献示例(内容基于典型研究领域概括，非真实文献)：
1. **文献名称**: *"Production and characterization of Fc-specific anti-rabbit IgG antibodies for immunoassays"*
**作者**: Smith J, et al.
**摘要**: 描述了通过免疫山羊制备兔IgG Fc段特异性抗体的方法，验证了其在ELISA和Western blot中对兔IgG重链Fc区域的高亲和力，且不与其他物种IgG发生交叉反应。
2. **文献名称**: *"Cross-reactivity analysis of anti-rabbit IgG Fc antibodies in multispecies studies"*
**作者**: Lee H, et al.
**摘要**: 评估了商品化兔IgG Fc特异性抗体与小鼠、人、大鼠IgG的交叉反应性，证明其在多重免疫检测中可特异性识别兔IgG，适用于血清样本分析。
3. **文献名称**: *"Application of Protein A/G-purified rabbit Fc-specific antibodies in blocking experiments"*
**作者**: Zhang R, et al.
**摘要**: 利用Protein A/G纯化的兔IgG Fc段抗体阻断非特异性Fc受体结合，优化了流式细胞术中免疫细胞的染色信噪比。
4. **文献名称**: *"Structural insights into rabbit IgG Fc recognition by monoclonal anti-Fc antibodies"*
**作者**: Brown K, et al.
**摘要**: 通过X射线晶体学解析了兔IgG Fc区域与特异性抗体的结合表位，揭示了重链CH2-CH3结构域的关键相互作用位点。
(注：以上为模拟生成的示例，实际引用需查询PubMed或学术数据库获取真实文献。)
Rabbit IgG (Fc-specific, Heavy Chain) antibodies are immuno-reagents designed to specifically target the constant region (Fc) of rabbit immunoglobulin G (IgG) heavy chains. IgG, the most abundant antibody class in serum, consists of two heavy chains and two light chains, with the Fc region located at the C-terminal end of the heavy chains. Fc-specific antibodies bind exclusively to this conserved Fc domain, avoiding interaction with the antigen-binding Fab regions. This specificity minimizes interference in assays where antigen-binding activity must remain unperturbed, such as in immune-complex detection or blocking applications.
These antibodies are widely used as secondary detection tools in techniques like Western blotting, ELISA, immunohistochemistry (IHC), and flow cytometry to identify rabbit-derived primary antibodies. Their Fc-specificity ensures recognition of IgG from rabbits regardless of the target antigen, making them versatile for diverse experimental setups. Additionally, they are employed in protein purification (e.g., via Protein A/G affinity columns) and immune response studies, particularly for analyzing Fc-mediated functions like complement activation or cellular effector interactions.
Produced typically in hosts like goats or donkeys, Fc-specific anti-rabbit IgG antibodies undergo cross-adsorption to eliminate cross-reactivity with immunoglobulins from other species (e.g., mouse, human), enhancing specificity. Their high affinity and tailored specificity make them critical reagents for reducing background noise and improving signal accuracy in multiplex assays or samples containing mixed antibody sources. This targeted design supports reproducible results in both research and diagnostic applications.
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