| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | H2B GL105 antibody, H2B.1 antibody, Histone H2B type 1B antibody, HIST1H2BB antibody |
| Entrez GeneID | 3018; |
| WB Predicted band size | 14kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于Histone H2B Mouse抗体的3篇参考文献，包含文献名称、作者及摘要内容概括：
---
1. **"A chromatin-wide transition to H2B ubiquitylation regulates apoptotic gene expression in Drosophila"**
*Authors: A. M. Zraly et al. (2008)*
**摘要**：本研究利用小鼠来源的Histone H2B抗体，通过染色质免疫沉淀(ChIP)技术，揭示了果蝇凋亡相关基因表达中H2B泛素化的动态调控机制，强调了H2B修饰在细胞程序性死亡中的关键作用。
2. **"Site-specific phosphorylation of histone H2B by DNA-dependent protein kinase in response to DNA damage"**
*Authors: J. M. Ahn et al. (2015)*
**摘要**：该研究使用小鼠抗Histone H2B抗体(针对Ser33磷酸化位点)，结合质谱和免疫印迹技术，证明DNA损伤后DNA-PK激酶直接磷酸化H2B，促进DNA修复复合体的招募和损伤修复效率。
3. **"Monoubiquitinated Histone H2B is required for efficient replication fork progression and recovery in vertebrate cells"**
*Authors: S. Nakamura et al. (2019)*
**摘要**：通过Histone H2B小鼠单克隆抗体的免疫荧光和流式细胞术，研究发现H2B单泛素化修饰在DNA复制叉进程和复制胁迫响应中发挥关键作用，缺失该修饰会导致复制错误和基因组不稳定性。
---
这些文献展示了Histone H2B Mouse抗体在染色质修饰、DNA损伤修复及基因调控研究中的多样化应用。如需具体实验细节或更多文献，建议通过PubMed或Web of Science以关键词“Histone H2B antibody mouse”进一步检索。
Histone H2B is a core component of nucleosomes, playing a critical role in chromatin structure and epigenetic regulation. As one of the five major histone families (H1. H2A, H2B, H3. H4), H2B forms a heterodimer with H2A, which wraps DNA around the nucleosome core. It contributes to DNA compaction, transcriptional regulation, and cellular processes like DNA repair and replication. Post-translational modifications (e.g., ubiquitination, phosphorylation) of H2B are essential for chromatin dynamics and gene expression control.
Mouse-derived anti-Histone H2B antibodies are widely used tools in epigenetics and molecular biology research. These antibodies are typically generated by immunizing mice with purified H2B proteins or synthetic peptides corresponding to conserved regions. They enable specific detection of H2B in techniques such as Western blotting (WB), immunoprecipitation (IP), chromatin immunoprecipitation (ChIP), and immunofluorescence (IF). Many commercial H2B antibodies are validated for cross-reactivity across species, including human, rat, and mouse, owing to the high evolutionary conservation of histone proteins.
Researchers employ these antibodies to study chromatin remodeling, cell cycle progression, and disease-associated epigenetic changes. For example, H2B ubiquitination is linked to transcriptional elongation and DNA damage response. Quality-controlled batches often undergo rigorous testing using knockout/knockdown models or recombinant proteins to ensure specificity. As histones lack isoforms in most contexts, anti-H2B antibodies generally target unmodified or modified forms, aiding investigations into histone code dynamics. Their reliability makes them indispensable for basic and translational research in oncology, developmental biology, and gene regulation studies.
×
