| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | BFP; EBFP; Blue Fluorescent Protein; Enhanced Blue Fluorescent Protein |
| Entrez GeneID | 7732; |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于EBFP小鼠抗体的3篇代表性文献摘要，供参考：
1. **《Development of a monoclonal antibody specific for enhanced blue fluorescent protein (EBFP) and its application in protein tagging studies》**
- **作者**: Johnson, R. et al.
- **摘要**: 本研究报道了一种针对EBFP的小鼠单克隆抗体的开发，通过免疫小鼠获得高亲和力抗体。该抗体在Western blot和免疫荧光中表现出特异性，成功用于追踪EBFP标记蛋白在哺乳动物细胞中的亚定位。
2. **《Characterization of anti-EBFP antibodies for improved detection in fusion protein systems》**
- **作者**: Chen, L. & Müller, J.
- **摘要**: 文章系统评估了多种小鼠源EBFP抗体的交叉反应性及灵敏度，优化了其在共聚焦显微镜下的成像条件，证明其适用于多色标记实验中减少与其他荧光蛋白的交叉干扰。
3. **《A novel EBFP-based biosensor screened by phage display-derived monoclonal antibodies》**
- **作者**: Sato, K. et al.
- **摘要**: 利用噬菌体展示技术筛选出高特异性小鼠抗EBFP抗体，并构建基于该抗体的FRET生物传感器，用于实时监测细胞内钙离子动态变化，拓展了EBFP在活细胞成像中的应用。
**注**：EBFP相关抗体文献较少，上述内容为模拟概括。实际研究中建议通过PubMed或Web of Science以关键词“EBFP mouse antibody”或“anti-EBFP monoclonal”检索最新文献，或关注常用抗体公司(如Thermo Fisher、Abcam)的技术资料引用文献。
**Background of EBFP Mouse Antibody**
The EBFP (Enhanced Blue Fluorescent Protein) Mouse antibody is a monoclonal or polyclonal antibody derived from mice, designed to specifically detect EBFP, a engineered variant of the original blue fluorescent protein (BFP). EBFP was developed through mutagenesis of green fluorescent protein (GFP) to shift its emission spectrum to the blue range (~440 nm), enabling its use as a genetically encoded reporter in molecular and cellular biology.
This antibody is typically generated by immunizing mice with purified EBFP or its immunogenic peptide sequences, followed by hybridoma technology (for monoclonal versions) or serum collection (for polyclonal forms). It exhibits high specificity for EBFP, often recognizing epitopes within the protein’s conserved structural regions or engineered motifs. EBFP antibodies are widely utilized in techniques such as Western blotting, immunofluorescence, flow cytometry, and immunoprecipitation to visualize and quantify EBFP-tagged proteins in vitro and in vivo.
Due to EBFP’s lower brightness and photostability compared to later-generation fluorescent proteins (e.g., EGFP, mCherry), its use has diminished over time. However, EBFP-specific antibodies remain valuable tools for validating historical experiments, troubleshooting cross-reactivity in multicolor imaging, or studying systems where EBFP-based constructs were originally implemented. Researchers often verify these antibodies for minimal cross-reactivity with other FP variants to ensure assay specificity.
In summary, EBFP Mouse antibodies serve as critical reagents for detecting EBFP fusion proteins, aiding in the study of gene expression, protein localization, and dynamic cellular processes.
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