| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Yellow florescent protein; Enhanced Yellow florescent protein; EYFP; YFP |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Formulation | Purified antibody in TBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于EYFP兔抗体的参考文献示例(注：以下文献信息为示例，实际引用时请核对真实文献来源)：
1. **文献名称**: "High-affinity rabbit monoclonal antibodies against EYFP for super-resolution imaging"
**作者**: Smith A, et al.
**摘要**: 本研究开发了高亲和力的兔单克隆抗体，特异性识别EYFP蛋白，适用于超分辨率显微镜技术。抗体在免疫荧光和免疫印迹中表现出低交叉反应性，成功用于活细胞中EYFP标记蛋白的纳米级定位。
2. **文献名称**: "Characterization of EYFP-tagged neuronal circuits using rabbit polyclonal antibodies"
**作者**: Johnson B, et al.
**摘要**: 利用兔多克隆抗体对转基因小鼠脑组织中EYFP标记的神经元回路进行免疫组化分析，验证了抗体在组织切片中的高特异性，为神经环路追踪提供了可靠工具。
3. **文献名称**: "Validation of anti-EYFP antibodies for quantitative flow cytometry applications"
**作者**: Lee C, et al.
**摘要**: 评估两种商品化兔源EYFP抗体的流式细胞检测性能，证实其在哺乳动物细胞系中可定量检测EYFP表达水平，灵敏度显著优于传统GFP抗体。
4. **文献名称**: "Cross-species reactivity of anti-EYFP antibodies in Drosophila and zebrafish models"
**作者**: Gupta R, et al.
**摘要**: 测试兔源EYFP抗体在果蝇和斑马鱼模型中的适用性，证明其能有效识别不同物种中EYFP融合蛋白，支持跨物种比较研究中的蛋白表达分析。
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**提示**：实际研究中，建议通过以下方式获取文献：
1. 在PubMed或Google Scholar搜索 **"EYFP antibody rabbit"** 或 **"anti-EYFP immunostaining"**。
2. 查阅抗体供应商(如Thermo Fisher、Abcam)官网提供的产品引用文献。
3. 关注领域内常用EYFP模型(如Thy1-EYFP转基因小鼠)的相关方法学论文。
The EYFP (Enhanced Yellow Fluorescent Protein) Rabbit antibody is a polyclonal or monoclonal antibody generated in rabbits against the EYFP, a genetically engineered variant of the green fluorescent protein (GFP). EYFP, derived from *Aequorea victoria* GFP through mutations such as S65G, V68L, Q69K, and T203Y, exhibits brighter fluorescence and a shifted emission spectrum toward yellow wavelengths (λ~530 nm). This modification enhances its utility in multicolor imaging and protein tagging applications.
The antibody is designed to specifically recognize EYFP-tagged fusion proteins in various experimental systems. It is widely used in techniques like Western blotting, immunofluorescence, immunohistochemistry, and flow cytometry to detect and localize EYFP-labeled proteins in cells, tissues, or transgenic organisms. Its high specificity minimizes cross-reactivity with other fluorescent proteins (e.g., GFP, CFP), ensuring reliable results in complex biological samples.
Produced by immunizing rabbits with purified recombinant EYFP, the antibody is typically affinity-purified to enhance specificity and reduce background noise. Its applications span neuroscience, cell biology, and molecular genetics, particularly in tracking protein dynamics, monitoring gene expression, or visualizing subcellular structures. Commercial EYFP antibodies often include validation data (e.g., knockout-validated specificity) to ensure reproducibility, making them critical tools for researchers leveraging fluorescent protein technologies.
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