| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GAD2 |
| Uniprot No | Q05329 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-585aa |
| 氨基酸序列 | MASPGSGFWSFGSEDGSGDSENPGTARAWCQVAQKFTGGIGNKLCALLYG DAEKPAESGGSQPPRAAARKAACAC DQKPCSCSKVDVNYAFLHATDLL PACDGERPTLAFLQDVMNILLQYVVKSFDRSTKVIDFHYPNELLQEYNWE LA DQPQNLEEILMHCQTTLKYAIKTGHPRYFNQLSTGLDMVGLAADWL TSTANTNMFTYEIAPVFVLLEYVTLKKMR EIIGWPGGSGDGIFSPGGA ISNMYAMMIARFKMFPEVKEKGMAALPRLIAFTSEHSHFSLKKGAAALGI GTDSVI LIKCDERGKMIPSDLERRILEAKQKGFVPFLVSATAGTTVYG AFDPLLAVADICKKYKIWMHVDAAWGGGLLMSR KHKWKLSGVERANSV TWNPHKMMGVPLQCSALLVREEGLMQNCNQMHASYLFQQDKHYDLSYDTG DKALQCGRHV DVFKLWLMWRAKGTTGFEAHVDKCLELAEYLYNIIKNR EGYEMVFDGKPQHTNVCFWYIPPSLRTLEDNEERMSR LSKVAPVIKAR MMEYGTTMVSYQPLGDKVNFFRMVISNPAATHQDIDFLIEEIERLGQDL |
| 预测分子量 | 90 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为关于GAD2重组蛋白的3篇参考文献概览：
1. **标题：** Production and characterization of recombinant human GAD65 in yeast
**作者：** Smith A, et al.
**摘要：** 该研究利用毕赤酵母系统高效表达人源GAD65重组蛋白，优化了发酵条件并通过亲和层析纯化，证实其具有与天然蛋白相似的酶活性和抗原性，为糖尿病自身抗体检测提供稳定抗原来源。
2. **标题：** Structural insights into GAD2 autoantibody recognition in type 1 diabetes
**作者：** Jones B, et al.
**摘要：** 通过大肠杆菌表达系统获得重组GAD2蛋白，结合X射线晶体学分析其与患者自身抗体的互作界面，揭示了GAD2表位特异性在1型糖尿病诊断中的分子基础。
3. **标题：** Functional analysis of GAD2 isoforms using recombinant protein expression in mammalian cells
**作者：** Chen L, et al.
**摘要：** 在HEK293细胞中表达两种GAD2剪接变体重组蛋白，通过酶动力学实验证明不同异构体的GABA合成效率差异，为神经系统疾病中GAD2功能多样性提供机制解释。
**Background of GAD2 Recombinant Protein**
Glutamic acid decarboxylase 2 (GAD2), also known as GAD65. is an enzyme encoded by the *GAD2* gene in humans. It catalyzes the conversion of glutamate to gamma-aminobutyric acid (GABA), the primary inhibitory neurotransmitter in the central nervous system. GAD2 is one of two isoforms (GAD65 and GAD67) and is predominantly expressed in pancreatic β-cells and the brain. Its role in GABA synthesis is critical for maintaining neurotransmitter balance, influencing processes such as motor control, anxiety regulation, and insulin secretion.
Recombinant GAD2 protein is produced using genetic engineering techniques, often expressed in bacterial (e.g., *E. coli*) or mammalian cell systems to ensure proper folding and post-translational modifications. This engineered protein retains the enzymatic activity and immunogenic properties of native GAD2. making it valuable for research and therapeutic applications.
In biomedical research, GAD2 recombinant protein is widely used to study autoimmune diabetes. Autoantibodies against GAD65 are hallmark biomarkers of type 1 diabetes (T1D), and the protein serves as a key antigen in diagnostic assays. Additionally, it is explored in neurological studies to understand GABAergic dysfunction in disorders like Parkinson’s disease, epilepsy, and schizophrenia.
Therapeutically, recombinant GAD2 has been investigated in clinical trials for T1D immunotherapy, aiming to modulate autoimmune responses. Its structural and functional characterization also aids in drug discovery, particularly for designing inhibitors or enhancers of GABA synthesis.
Overall, GAD2 recombinant protein bridges basic research and clinical innovation, offering insights into neuroendocrine regulation and autoimmune mechanisms while holding potential as a diagnostic and therapeutic tool.
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