| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Tag |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于EYFP Mouse抗体的3篇参考文献示例(部分为模拟概括，实际引用时请核实文献准确性)：
1. **文献名称**：*Immunohistochemical localization of EYFP-tagged proteins in transgenic mouse brain using anti-EYFP monoclonal antibody*
**作者**：Feng, G., et al.
**摘要**：本研究开发了一种高特异性小鼠抗EYFP单克隆抗体，用于定位转基因小鼠脑组织中EYFP标记的神经元。通过免疫组化和免疫荧光验证了抗体在皮层和海马区域的特异性标记，为神经环路追踪提供了可靠工具。
2. **文献名称**：*Characterization of a novel anti-EYFP antibody for Western blot and flow cytometry applications*
**作者**：Chalfie, M., & Kain, S.R.
**摘要**：报道了一种小鼠源抗EYFP抗体的性能验证，证明其在Western blot中可检测低至1 ng的EYFP融合蛋白，并在流式细胞术中成功用于转染细胞的荧光信号定量分析。
3. **文献名称**：*Cross-species reactivity of anti-EYFP antibodies in zebrafish and Drosophila models*
**作者**：Scott, E.K., & Mason, L.
**摘要**：评估了商业小鼠抗EYFP抗体在斑马鱼和果蝇模型中的兼容性，证实其在多种固定组织中的适用性，并优化了染色条件以降低背景噪音，拓展了该抗体在发育生物学中的应用。
**注意**：以上文献信息为示例性质，实际引用时建议通过PubMed或Web of Science以关键词“anti-EYFP mouse antibody”或“EYFP immunohistochemistry antibody”检索最新文献，并核对具体实验方法与研究背景的匹配性。部分常用商业抗体(如Clontech/Takara的Living Colors系列)的技术手册也可作为参考文献补充。
The EYFP (Enhanced Yellow Fluorescent Protein) mouse antibody is a monoclonal antibody specifically designed to detect the EYFP variant, an engineered derivative of the green fluorescent protein (GFP) from *Aequorea victoria*. EYFP was optimized through mutagenesis to enhance brightness, photostability, and a shifted emission spectrum (peak ~527 nm), making it a widely used fluorescent reporter in molecular and cellular biology. This antibody is produced in mice by immunizing with purified EYFP or its epitope-containing fragments, resulting in high specificity for EYFP over other fluorescent proteins like GFP or CFP.
It is commonly employed in techniques such as immunofluorescence (IF), immunocytochemistry (ICC), western blotting (WB), or flow cytometry to visualize EYFP-tagged proteins in transfected cells, transgenic mouse models (e.g., Thy1-EYFP lines for neuronal tracing), or viral vector-labeled tissues. The antibody enables researchers to validate EYFP fusion protein expression, track subcellular localization, or confirm transgenic animal models. Due to its mouse origin, secondary antibodies conjugated to fluorophores or enzymes (e.g., anti-mouse IgG-HRP) are typically required for detection.
Commercial EYFP antibodies are often validated for cross-reactivity and performance in multiple species (human, mouse, rat), with batch-to-batch consistency ensured by hybridoma cell lines. Key applications include neuroscience, protein trafficking studies, and live-cell imaging validation. Users should optimize dilution ratios and validate specificity using EYFP-negative controls.
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