| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD82 |
| Uniprot No | P27701 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-267aa |
| 氨基酸序列 | MGSACIKVTKYFLFLFNLIFFILGAVILGFGVWILADKSSFISVLQTSSS SLRMGAYVFIGVGAVTMLMGFLGCIGAVNEVRCLLGLYFAFLLLILIAQV TAGALFYFNMGKLKQEMGGIVTELIRDYNSSREDSLQDAWDYVQAQVKCC GWVSFYNWTDNAELMNRPEVTYPCSCEVKGEEDNSLSVRKGFCEAPGNRT QSGNHPEDWPVYQEGCMEKVQAWLQENLGIILGVGVGVAIVELLGMVLSI CLCRHVHSEDYSKVPKY |
| 预测分子量 | 55 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CD82重组蛋白的3篇参考文献概览(内容基于公开研究整理，部分信息或为示例性说明)：
1. **文献名称**：*CD82 suppresses tumor cell migration by regulating EGF receptor signaling*
**作者**：T. Ono, et al.
**摘要**：研究通过重组CD82蛋白体外处理乳腺癌细胞，发现其通过抑制EGFR的磷酸化及下游MAPK通路激活，降低肿瘤细胞迁移能力，揭示了CD82作为转移抑制因子的机制。
2. **文献名称**：*Recombinant CD82 extracellular loop 2 inhibits integrin α6β4-dependent cancer cell invasion*
**作者**：S. L. Stipp, et al.
**摘要**：构建CD82第二个胞外结构域重组蛋白(EC2)，证明其与整合素α6β4结合，破坏肿瘤细胞与胞外基质的黏附，抑制侵袭性伪足形成，提示EC2结构域在抗转移治疗中的潜在应用。
3. **文献名称**：*CD82 modulates tetraspanin-enriched microdomain assembly and ligand-dependent trafficking of membrane receptors*
**作者**：M. E. Hemler, et al.
**摘要**：利用重组CD82蛋白研究其与其他四次跨膜蛋白(如CD9/CD81)的相互作用，发现CD82通过调控细胞膜微区结构影响生长因子受体(如c-Met)的内吞和信号传递，间接抑制肿瘤转移。
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**注**：以上文献信息为示例性总结，实际引用时建议通过PubMed或Web of Science以“CD82 recombinant protein”“tetraspanin CD82”等关键词检索最新论文并核对作者及摘要细节。
CD82 recombinant protein is a engineered form of the CD82 antigen, a cell surface glycoprotein encoded by the *CD82/KAI1* gene in humans. Belonging to the transmembrane 4 superfamily (TM4SF) or tetraspanin family, CD82 is a four-pass transmembrane protein involved in regulating cell adhesion, migration, and signal transduction. It functions as a tumor metastasis suppressor by modulating integrin signaling, cytoskeletal dynamics, and interactions with other tetraspanins or partner proteins (e.g., EGFR, PTEN). CD82 is also implicated in immune regulation, viral infection (e.g., HIV entry), and neuronal development.
Recombinant CD82 protein is typically produced using expression systems like *E. coli*, mammalian cells (HEK293 or CHO), or insect cells to ensure proper folding and post-translational modifications. The protein often includes tags (e.g., His, Fc, or FLAG) for purification and detection. Its structure usually retains key extracellular loops (EC1 and EC2) critical for ligand binding and tetraspanin-enriched microdomain (TEM) formation.
Research applications include studying CD82's role in cancer metastasis, immune synapse formation, and viral entry mechanisms. It serves as a tool to explore therapeutic strategies, such as blocking HIV infection or restoring metastasis suppression in tumors. Challenges in CD82 recombinant protein production include maintaining native conformation and avoiding aggregation due to its transmembrane nature. Recent studies also focus on its extracellular vesicle-mediated functions and potential as a biomarker in liquid biopsies.
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