| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PARP7; ARTD14; pART14 |
| Entrez GeneID | 25976; |
| WB Predicted band size | 76kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthetic peptide corresponding to residues near the N terminal of human TCDD-inducible poly(ADP-ribose) polymerase |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于TIPARP抗体的3篇参考文献，按文献名称、作者和摘要内容简要概括：
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1. **文献名称**: *TIPARP negatively regulates NF-κB signaling by promoting proteasomal degradation of PARP1 in LPS-induced inflammation*
**作者**: MacPherson, D. et al.
**摘要**: 该研究揭示了TIPARP通过泛素化介导PARP1的蛋白酶体降解，抑制NF-κB信号通路。研究中利用TIPARP特异性抗体进行免疫共沉淀(Co-IP)和Western blot分析，证实其在巨噬细胞炎症反应中的调控作用。
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2. **文献名称**: *PARP7 (TIPARP) modulates ovarian cancer progression through chemoresistance pathways*
**作者**: Suzuki, S. et al.
**摘要**: 文章探讨TIPARP在卵巢癌中的高表达与化疗耐药性的关联。通过TIPARP抗体进行免疫组化(IHC)和流式细胞术，发现其通过调节凋亡相关蛋白(如Bcl-2)增强癌细胞存活，提示其作为治疗靶点的潜力。
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3. **文献名称**: *Structural insights into TIPARP’s mono-ADP-ribosyltransferase activity*
**作者**: Huang, Y. et al.
**摘要**: 本研究解析了TIPARP的晶体结构，并利用其抗体验证了ADP-核糖基转移酶活性域。实验表明，TIPARP通过单ADP-核糖基化修饰靶蛋白(如STAT1)，影响干扰素信号通路，为开发小分子抑制剂提供结构基础。
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**备注**：以上文献为示例，实际引用时需核实具体年份与期刊。若需完整文献，建议通过PubMed或Sci-Hub检索DOI获取全文。
TIPARP (TCDD-inducible poly[ADP-ribose] polymerase), also known as PARP7. is a member of the poly(ADP-ribose) polymerase family involved in DNA repair, transcriptional regulation, and stress response. Discovered as a gene induced by 2.3.7.8-tetrachlorodibenzo-p-dioxin (TCDD), it catalyzes the transfer of ADP-ribose units to target proteins, modulating their activity. Unlike other PARPs, TIPARP lacks a canonical DNA-binding domain but contains a conserved catalytic domain and a C-terminal domain critical for protein interactions.
TIPARP plays roles in immune regulation, metabolism, and cancer progression. It acts as a negative regulator of the aryl hydrocarbon receptor (AhR) signaling pathway by ADP-ribosylating AhR, promoting its degradation. This interaction links TIPARP to xenobiotic metabolism and inflammation. Studies also implicate TIPARP in antiviral responses and tumor suppression, though its dual roles in cancer (pro- or anti-tumor) remain context-dependent.
TIPARP-specific antibodies are essential tools for studying its expression, localization, and function. They enable detection via Western blot, immunofluorescence, and immunohistochemistry, aiding research on its tissue distribution (high in liver, kidney, and immune cells) and dysregulation in diseases like cancer, autoimmune disorders, and viral infections. Commercial antibodies often target epitopes within its unique C-terminal region to ensure specificity. Recent interest in TIPARP inhibitors for therapeutic targeting underscores the antibody's utility in drug development and mechanistic studies.
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