| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CNTN4 |
| Uniprot No | Q8IWV2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1026aa |
| 氨基酸序列 | MRLPWELLVLQSFILCLADDSTLHGPIFIQEPSPVMFPLDSEEKKVKLNCEVKGNPKPHIRWKLNGTDVDTGMDFRYSVVEGSLLINNPNKTQDAGTYQCTATNSFGTIVSREAKLQFAYLDNFKTRTRSTVSVRRGQGMVLLCGPPPHSGELSYAWIFNEYPSYQDNRRFVSQETGNLYIAKVEKSDVGNYTCVVTNTVTNHKVLGPPTPLILRNDGVMGEYEPKIEVQFPETVPTAKGATVKLECFALGNPVPTIIWRRADGKPIARKARRHKSNGILEIPNFQQEDAGLYECVAENSRGKNVARGQLTFYAQPNWIQKINDIHVAMEENVFWECKANGRPKPTYKWLKNGEPLLTRDRIQIEQGTLNITIVNLSDAGMYQCLAENKHGVIFSNAELSVIAVGPDFSRTLLKRVTLVKVGGEVVIECKPKASPKPVYTWKKGRDILKENERITISEDGNLRIINVTKSDAGSYTCIATNHFGTASSTGNLVVKDPTRVMVPPSSMDVTVGESIVLPCQVTHDHSLDIVFTWSFNGHLIDFDRDGDHFERVGGQDSAGDLMIRNIQLKHAGKYVCMVQTSVDRLSAAADLIVRGPPGPPEAVTIDEITDTTAQLSWRPGPDNHSPITMYVIQARTPFSVGWQAVSTVPELIDGKTFTATVVGLNPWVEYEFRTVAANVIGIGEPSRPSEKRRTEEALPEVTPANVSGGGGSKSELVITWETVPEELQNGRGFGYVVAFRPYGKMIWMLTVLASADASRYVFRNESVHPFSPFEVKVGVFNNKGEGPFSPTTVVYSAEEEPTKPPASIFARSLSATDIEVFWASPLEKNRGRIQGYEVKYWRHEDKEENARKIRTVGNQTSTKITNLKGSVLYHLAVKAYNSAGTGPSSATVNVTTRKPPPSQPPGNIIWNSSDSKIILNWDQVKALDNESEVKGYKVLYRWNRQSSTSVIETNKTSVELSLPFDEDYIIEIKPFSDGGDGSSSEQIRIPKISNAYARGSGASTSNACTLSAISTIMISLTARSSL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CNTN4重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*CNTN4 as a novel candidate gene for neurodevelopmental disorders*
**作者**：Smith A, et al.
**摘要**：研究通过重组CNTN4蛋白在体外模型中验证其与突触蛋白的相互作用，发现CNTN4缺失影响神经元轴突导向，提示其与自闭症谱系障碍的潜在关联。
2. **文献名称**：*Expression and functional characterization of recombinant Contactin-4 in glioblastoma cells*
**作者**：Zhang L, et al.
**摘要**：成功在大肠杆菌中表达并纯化重组CNTN4蛋白，实验显示其可抑制胶质瘤细胞迁移，为靶向治疗提供新思路。
3. **文献名称**：*Structural analysis of CNTN4-Fc fusion protein and its role in neural circuit formation*
**作者**：Tanaka K, et al.
**摘要**：构建CNTN4-Fc重组融合蛋白，解析其晶体结构，证实其通过结合APP(淀粉样前体蛋白)调控海马神经元网络发育。
(注：以上文献信息为示例性质，实际引用需核对具体论文数据。)
Contactin-4 (CNTN4), a member of the contactin family of cell adhesion molecules, plays a critical role in neural development and synaptic plasticity. This glycosylphosphatidylinositol (GPI)-anchored protein is primarily expressed in the central nervous system, where it facilitates cell-cell interactions by binding to extracellular matrix components or receptors on adjacent cells. Structurally, CNTN4 consists of six immunoglobulin-like domains and four fibronectin type III repeats, which mediate its adhesive functions. It is implicated in axon guidance, neurite outgrowth, and the formation of neuronal networks, particularly in regions associated with cognitive and sensory processing.
Recombinant CNTN4 protein is engineered through heterologous expression systems (e.g., mammalian, insect, or HEK293 cells) to ensure proper post-translational modifications. This purified protein retains functional domains, enabling researchers to study its interactions in vitro. Applications include investigating CNTN4's role in neurodevelopmental disorders, as genomic studies link CNTN4 deletions or mutations to autism spectrum disorders (ASD) and intellectual disability. Additionally, CNTN4 dysregulation has been observed in cancers, suggesting potential involvement in tumor metastasis via cell adhesion pathways.
Beyond neuroscience, recombinant CNTN4 serves as a tool for developing diagnostic biomarkers or therapeutic targets. Its ability to modulate neuronal connectivity makes it valuable for modeling disease mechanisms or screening compounds aimed at restoring neural function. However, functional redundancy within the contactin family and context-dependent signaling effects necessitate careful experimental design. Ongoing research continues to unravel CNTN4's multifaceted roles, bridging gaps between structural biology, neuropathology, and translational medicine.
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