| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Geph |
| Entrez GeneID | 64845; |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthetic peptide corresponding to a region derived from internal residues of human gephyrin |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于GPHN(Gephyrin)抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*Gephyrin autoantibodies in a patient with stiff-person syndrome and breast cancer*
**作者**：Pfeiffer et al.
**摘要**：该研究报道了一名僵人综合征(SPS)合并乳腺癌患者的血清中检测到GPHN自身抗体，揭示了Gephyrin抗体可能与自身免疫性神经系统疾病及副肿瘤综合征相关，提示其作为临床诊断标志物的潜在价值。
2. **文献名称**：*Structural insights into the role of Gephyrin in inhibitory synapse organization*
**作者**：Sola et al.
**摘要**：通过X射线晶体学解析Gephyrin蛋白结构，阐明其通过寡聚化形成支架网络调控抑制性突触受体(如GABA_A和甘氨酸受体)的聚集。研究中使用特异性GPHN抗体进行免疫荧光定位，验证了其在突触后膜的功能。
3. **文献名称**：*Gephyrin mutations lead to neurodevelopmental defects and epilepsy in mice*
**作者**：Yu et al.
**摘要**：在小鼠模型中敲除Gephyrin基因导致抑制性突触功能异常，引发癫痫和认知障碍。实验通过Western blot和免疫组化(使用GPHN抗体)证实突变体中蛋白表达缺失，为研究癫痫发病机制提供了分子基础。
(注：以上文献标题和内容为概括性示例，实际文献需通过数据库如PubMed检索确认。)
**Background of GPHN Antibody**
Gephyrin (GPHN) is a multifunctional protein critical for the formation and maintenance of inhibitory synapses in the central nervous system. It acts as a scaffold protein at post-synaptic sites, anchoring glycine and GABAA receptors to the cytoskeleton by interacting with microtubules and binding partners like collybistin. GPHN also plays roles in molybdenum cofactor biosynthesis, linking its function to metabolic pathways.
Antibodies targeting GPHN are essential tools in neuroscience research, enabling the study of synaptic organization, receptor clustering, and plasticity. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to visualize GPHN expression, localization, and interactions in neuronal tissues.
Dysregulation of GPHN is associated with neurological disorders, including epilepsy, autism spectrum disorders, and schizophrenia. Research using GPHN antibodies has revealed its involvement in synaptic pathology, such as altered receptor distribution or synaptic density in disease models. Additionally, studies explore post-translational modifications (e.g., phosphorylation) of GPHN that modulate its scaffolding activity.
These antibodies often target specific domains (e.g., N-terminal or C-terminal regions) or modified epitopes, aiding in mechanistic studies of synaptic dysfunction. Their applications extend to drug discovery, where GPHN-based assays help identify compounds targeting inhibitory neurotransmission. Overall, GPHN antibodies are pivotal in advancing understanding of synaptic biology and neurological disease mechanisms.
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