| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PIKE; GGAP2; CENTG1 |
| Entrez GeneID | 116986; |
| WB Predicted band size | 125kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide corresponding to residues near the C terminal of human ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于AGAP2抗体的3篇参考文献的简要信息：
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1. **文献名称**：*AGAP2 promotes glioblastoma proliferation, invasion, and tumorigenicity via NF-κB signaling*
**作者**：Li Y, et al.
**摘要**：该研究利用AGAP2抗体通过Western blot和免疫组化技术，发现AGAP2在胶质母细胞瘤中高表达，并通过激活NF-κB通路促进肿瘤细胞增殖和侵袭。
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2. **文献名称**：*Role of AGAP2 in Hepatocellular Carcinoma Metastasis and its Clinical Implications*
**作者**：Wang X, et al.
**摘要**：研究者通过免疫荧光和AGAP2抗体检测肝癌组织样本，证实AGAP2过表达与患者预后不良相关，并调控了EMT(上皮间质转化)过程。
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3. **文献名称**：*AGAP2 interacts with EGFR to regulate breast cancer progression via PI3K/AKT pathway*
**作者**：Chen L, et al.
**摘要**：该研究使用AGAP2抗体进行共免疫沉淀(Co-IP)和免疫组化分析，揭示了AGAP2与EGFR的结合在乳腺癌PI3K/AKT通路激活中的关键作用。
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**注**：以上文献为示例性概括，实际文献需通过PubMed、Google Scholar等数据库检索确认。建议以“AGAP2 antibody”或“AGAP2 (ArfGAP2)”为关键词查找近年研究。
The AGAP2 (ArfGAP with GTPase domain, ANK repeat, and PH domain 2) antibody is a research tool used to detect and study the AGAP2 protein, a member of the ArfGAP (ADP-ribosylation factor GTPase-activating protein) family. AGAP2 contains three functional domains: a GTPase-activating protein (GAP) domain that regulates Arf GTPases, ankyrin (ANK) repeats for protein-protein interactions, and a pleckstrin homology (PH) domain for membrane binding. This protein plays roles in membrane trafficking, cytoskeletal reorganization, and cell signaling by modulating Arf-mediated vesicle formation and receptor trafficking.
AGAP2 is implicated in various cellular processes, including endosomal sorting, receptor recycling, and neuronal development. Dysregulation of AGAP2 has been linked to cancer progression, particularly in glioblastoma, breast cancer, and prostate cancer, where it may promote cell proliferation, migration, and invasion via pathways like PI3K/AKT/mTOR. It also interacts with signaling molecules such as β-arrestin and APPL1. suggesting roles in receptor tyrosine kinase signaling and apoptosis.
Antibodies targeting AGAP2 are used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to explore its expression, localization, and interactions. These tools help elucidate AGAP2’s pathophysiological roles, offering insights into therapeutic targeting in oncology and neurodegenerative disorders. Validation of AGAP2 antibodies is critical to ensure specificity, given structural similarities among ArfGAP family members.
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