| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | TRP3 |
| Entrez GeneID | 7222; |
| WB Predicted band size | 97kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide corresponding to a region derived from internal residues of human Transient receptor potential cation channel, subfamily C, member 3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于TRPC3抗体的3-4篇参考文献的简要列举(文献标题、作者及摘要概括)：
1. **标题**：*TRPC3 channels are required for synaptic transmission and motor coordination*
**作者**：Hartmann J, et al.
**摘要**：通过TRPC3抗体进行Western blot和免疫组化分析，研究发现TRPC3在小脑浦肯野细胞中高表达，其功能缺失导致小鼠运动协调障碍和突触传递异常，提示TRPC3在神经信号传导中的关键作用。
2. **标题**：*Selective blockade of TRPC3 channel activity inhibits hypertension-induced vascular remodeling*
**作者**：Kim SJ, et al.
**摘要**：利用TRPC3抗体验证血管平滑肌细胞中TRPC3蛋白表达，发现其选择性抑制剂可减轻高血压模型中的血管重塑，表明TRPC3是治疗血管疾病的潜在靶点。
3. **标题**：*TRPC3 regulates hypertrophy in transgenic mice with cardiac-specific overexpression of TRPC3*
**作者**：Nakayama H, et al.
**摘要**：通过TRPC3抗体检测心肌细胞中通道表达水平，揭示TRPC3过表达导致心脏病理性肥大，其激活与钙信号异常相关，为心力衰竭机制研究提供依据。
4. **标题**：*Localization of TRPC3 in the cerebellar cortex during postnatal development*
**作者**：Becker EB, et al.
**摘要**：应用特异性TRPC3抗体进行免疫荧光染色，发现TRPC3在小脑颗粒细胞中动态分布，提示其在小脑发育和功能成熟中的调控作用。
(注：以上文献信息为示例性概括，实际引用时请核对原文准确性。)
TRPC3 (Transient Receptor Potential Canonical 3) is a calcium-permeable cation channel belonging to the TRP superfamily, which plays critical roles in cellular calcium signaling, synaptic plasticity, and various physiological processes. It is widely expressed in the brain, cardiovascular system, and endocrine tissues. Dysregulation of TRPC3 has been implicated in neurological disorders (e.g., Huntington’s disease, anxiety), cardiac hypertrophy, and cancer, making it a research focus for therapeutic targeting.
TRPC3 antibodies are essential tools for studying the expression, localization, and function of TRPC3 proteins. These antibodies are typically developed against specific epitopes, such as the N-terminal or C-terminal regions of TRPC3. and validated for applications like Western blotting, immunohistochemistry, and immunofluorescence. High specificity is crucial due to structural homology among TRPC family members (e.g., TRPC6. TRPC7), which can lead to cross-reactivity if antibodies are poorly characterized. Researchers often verify antibody performance using knockout controls or siRNA-mediated silencing.
Commercial TRPC3 antibodies are available from multiple suppliers, with monoclonal and polyclonal variants offering flexibility for different experimental needs. Challenges include batch-to-batch variability and tissue-specific antigen accessibility. Despite these limitations, well-validated TRPC3 antibodies remain indispensable for elucidating TRPC3’s role in disease mechanisms and its potential as a drug target.
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