| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | SSeCKS; AKAP250 |
| Entrez GeneID | 9590; |
| WB Predicted band size | 191kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide corresponding to residues near the C terminal of human A kinase (PRKA) anchor protein 12 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于AKAP12抗体的3-4篇示例文献(内容为示例，建议通过学术数据库验证具体信息)：
---
1. **文献名称**：*AKAP12 as a tumor suppressor and metastasis inhibitor in human cancers*
**作者**：Kang et al. (2005)
**摘要**：研究报道AKAP12通过调控细胞骨架和信号通路抑制肿瘤转移，使用AKAP12抗体进行Western blot和免疫组化，发现其在多种癌症中表达下调。
2. **文献名称**：*AKAP12 modulates integrin signaling and cancer cell motility*
**作者**：Xie et al. (2010)
**摘要**：探讨AKAP12通过整合素通路抑制癌细胞迁移的机制，利用AKAP12抗体进行免疫共沉淀和共聚焦显微镜分析其与信号复合物的相互作用。
3. **文献名称**：*Dynamic localization of AKAP12 in angiogenesis regulation*
**作者**：Lee et al. (2018)
**摘要**：研究AKAP12在血管生成中的动态定位，通过AKAP12抗体结合免疫荧光技术揭示其在内皮细胞中的亚细胞分布及功能。
4. **文献名称**：*Phosphorylation-dependent regulation of AKAP12 in cell cycle progression*
**作者**：Chen et al. (2020)
**摘要**：分析AKAP12磷酸化状态对细胞周期的影响，采用AKAP12特异性抗体进行流式细胞术和免疫印迹，揭示其与CDK1的调控关系。
---
**注**：以上文献信息为示例，实际引用需通过PubMed、Google Scholar等平台核实作者、年份及摘要准确性。AKAP12抗体的应用常见于蛋白表达检测(如Western blot)、亚细胞定位(免疫荧光)及相互作用研究(免疫共沉淀)等场景。
The AKAP12 antibody is a crucial tool for studying the A-kinase anchoring protein 12 (AKAP12), a scaffold protein involved in coordinating signaling complexes that regulate cell cycle progression, migration, and angiogenesis. AKAP12. also known as gravin or SSeCKS, binds to protein kinase A (PKA), protein kinase C (PKC), and other signaling molecules, facilitating compartmentalized phosphorylation events and signal transduction. It plays roles in maintaining endothelial barrier integrity, suppressing tumor metastasis, and modulating vascular remodeling by interacting with partners like β-arrestin and phosphodiesterases.
AKAP12 antibodies are widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to detect protein expression, localization, and post-translational modifications (e.g., phosphorylation) across tissues and cell lines. Dysregulation of AKAP12 has been linked to cancers, cardiovascular diseases, and fibrosis, making these antibodies valuable for mechanistic and diagnostic research. Commercial AKAP12 antibodies are typically raised against specific epitopes (e.g., human N-terminal residues 1-300) and validated for cross-reactivity in models like mouse, rat, or zebrafish, where homologs share high sequence conservation. Researchers must verify antibody specificity using knockout controls due to potential isoform variability (e.g., AKAP12A vs. AKAP12B) and tissue-specific splicing.
×
