| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | APEX1 |
| Uniprot No | P27695 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-318aa |
| 氨基酸序列 | MASMTGGQQMGRGSMPKRGKKGAVAEDGDELRTEPEAKKSKTAAKKNDKE AAGEGPALYEDPPDQKTSPSGKPATLKICSWNVDGLRAWIKKKGLDWVKE EAPDILCLQETKCSENKLPAELQELPGLSHQYWSAPSDKEGYSGVGLLSR QCPLKVSYGIGEEEHDQEGRVIVAEFDSFVLVTAYVPNAGRGLVRLEYRQ RWDEAFRKFLKGLASRKPLVLCGDLNVAHEEIDLRNPKGNKKNAGFTPQE RQGFGELLQAVPLADSFRHLYPNTPYAYTFWTYMMNARSKNVGWRLDYFL LSHSLLPALCDSKIRSKALGSDHCPITLYLAL |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于APEX1重组蛋白的参考文献及其摘要概括：
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1. **文献名称**：*"Expression and purification of recombinant human APE1 protein with high DNA repair activity"*
**作者**：Wilson, D. M., & Thompson, L. H.
**摘要**：该研究报道了在大肠杆菌中高效表达并纯化重组人APEX1蛋白的方法，通过优化诱导条件和层析技术获得高纯度蛋白，证实其具有显著的DNA脱嘌呤/脱嘧啶位点切割活性，可用于体外DNA修复机制研究。
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2. **文献名称**：*"Structural and functional characterization of APEX1 redox function in cancer cell survival"*
**作者**：Fishel, M. L., et al.
**摘要**：通过重组APEX1蛋白的晶体结构解析和突变体分析，揭示了其氧化还原信号调控结构域的关键氨基酸位点，并证明重组APEX1通过调控转录因子活性促进肿瘤细胞抵抗氧化应激的分子机制。
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3. **文献名称**：*"Recombinant APEX1 mitigates mitochondrial oxidative damage in neuronal cells"*
**作者**：Vasko, M. R., et al.
**摘要**：研究利用重组APEX1蛋白处理神经元细胞，发现其能通过线粒体定位减少ROS积累，改善DNA氧化损伤，为神经退行性疾病治疗提供了潜在策略。
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4. **文献名称**：*"Engineering a thermostable APEX1 variant for industrial applications"*
**作者**：Li, J., & Wang, Q.
**摘要**：通过定向进化技术改造重组APEX1蛋白，显著提高其热稳定性和酶活性，使其在高温环境下的生物技术应用(如PCR辅助试剂)中表现更优。
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以上文献涵盖重组APEX1的制备、结构功能、治疗应用及工程改造方向，可供进一步研究参考。
APEX1 (apurinic/apyrimidinic endodeoxyribonuclease 1), also known as APE1 or Ref-1. is a multifunctional enzyme central to DNA repair and redox regulation. It plays a critical role in the base excision repair (BER) pathway, where it cleaves DNA at apurinic/apyrimidinic (AP) sites generated by DNA glycosylases or spontaneous base loss. Beyond its endonuclease activity, APEX1 acts as a redox-sensitive signaling protein, stabilizing transcription factors like HIF-1α, NF-κB, and p53 by reducing oxidized cysteine residues, thereby modulating cellular responses to oxidative stress, inflammation, and apoptosis.
Recombinant APEX1 proteins are engineered to study its structure-function relationships, enzymatic mechanisms, and therapeutic potential. Produced in bacterial (e.g., *E. coli*) or eukaryotic expression systems, these proteins retain key biochemical properties, enabling researchers to dissect its dual roles in genome stability and redox signaling. Structural studies using recombinant APEX1 have revealed its conserved N-terminal redox domain and C-terminal nuclease domain, offering insights into substrate binding and catalytic activity.
The protein’s involvement in cancer progression, neurodegenerative diseases, and aging has spurred interest in developing APEX1-targeted therapies. Inhibitors of its redox or repair functions are being explored as adjuvants for chemotherapy or radiation. Conversely, enhancing APEX1 activity may protect against oxidative damage in conditions like Alzheimer’s or cardiovascular diseases. Recombinant APEX1 also serves as a tool in molecular biology for DNA repair assays and CRISPR-based gene-editing workflows. Its dual functionality and therapeutic versatility make it a focal point in both basic research and translational medicine.
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