| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MTHFR |
| Entrez GeneID | 4524 |
| clone | 5D3 |
| WB Predicted band size | 75kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | Purified recombinant fragment of human MTHFR expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
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以下是关于SLC22A8(OAT3)抗体的参考文献示例，包含文献名称、作者及摘要内容概括：
1. **"Localization of Organic Anion Transporter 3 (OAT3) in Rodent Kidney: A Comparative Study"**
**作者**: Vanwert AL, et al.
**摘要**: 使用SLC22A8特异性抗体进行免疫组化分析，揭示OAT3在小鼠和大鼠肾脏近端小管基底膜的表达模式，支持其在有机阴离子药物排泄中的关键作用。
2. **"Downregulation of OAT3 in Diabetic Nephropathy: Implications for Uremic Toxin Retention"**
**作者**: Hosoyamada M, et al.
**摘要**: 通过Western blot和免疫荧光技术(基于SLC22A8抗体)，发现糖尿病肾病模型中OAT3蛋白表达显著降低，提示其与尿毒症毒素蓄积的病理关联。
3. **"Functional Characterization of OAT3 Polymorphisms Using Antibody-Based Detection in Human Cell Lines"**
**作者**: Tzvetkov MV, et al.
**摘要**: 利用SLC22A8抗体验证转染细胞中OAT3蛋白的表达水平，结合功能实验证实基因多态性对其转运活性的影响，为个体化用药提供依据。
4. **"Sex-Specific Regulation of Renal OAT3 Expression by Hormones"**
**作者**: Cheng Y, et al.
**摘要**: 采用SLC22A8抗体进行免疫印迹和免疫组化，发现雌激素通过调控OAT3表达影响药物肾脏清除率的性别差异，为药代动力学研究提供新视角。
*注：以上文献信息为示例性内容，实际引用时需核实原文准确性及数据库收录情况。建议通过PubMed或Web of Science以关键词“SLC22A8 antibody”或“OAT3 antibody”检索最新研究。*
The SLC22A8 antibody is a research tool designed to detect and analyze the organic anion transporter 3 (OAT3), encoded by the *SLC22A8* gene. OAT3. a member of the solute carrier (SLC) 22 family, is a critical transmembrane protein involved in the transport of endogenous metabolites, xenobiotics, and drugs, particularly organic anions. It is predominantly expressed in the kidneys (basolateral membrane of proximal tubules), liver, and brain, playing a key role in renal excretion, drug pharmacokinetics, and toxin clearance. OAT3 mediates the uptake of substrates such as antibiotics, antivirals, NSAIDs, and uremic toxins, making it essential in studies of drug-drug interactions, nephrotoxicity, and metabolic diseases.
The SLC22A8 antibody is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study OAT3 expression, localization, and regulation under physiological or pathological conditions (e.g., chronic kidney disease, hyperuricemia). Researchers also employ this antibody to explore OAT3's role in hormonal signaling (e.g., handling of cortisol, prostaglandins) and its interaction with signaling pathways like MAPK/ERK. Validated antibodies show specificity for human, mouse, or rat OAT3. aiding translational research. However, batch variability and cross-reactivity with related transporters (e.g., OAT1) require careful validation via knockout models or siRNA knockdown. Its applications extend to drug development and toxicology, highlighting its importance in understanding membrane transport mechanisms and personalized medicine.
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