| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 7B4; CD144 |
| Entrez GeneID | 1003; |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide corresponding to residues near the C terminal of human cadherin 5, type 2 (vascular endothelium) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是模拟生成的3篇关于CDH5抗体的参考文献示例(非真实文献，仅供格式参考)：
1. **文献名称**: "VE-cadherin (CDH5) antibody characterization in endothelial permeability studies"
**作者**: Smith J et al.
**摘要**: 本研究验证了CDH5抗体在体外内皮细胞模型中的特异性，证明其可用于检测血管通透性变化，并发现TNF-α炎症刺激下CDH5蛋白表达的下调与细胞连接破坏相关。
2. **文献名称**: "CDH5 as a biomarker for tumor angiogenesis: Validation of a novel monoclonal antibody"
**作者**: Lee H et al.
**摘要**: 开发了一种高特异性CDH5单克隆抗体(克隆号#4B7)，通过免疫荧光和流式细胞术证实其可标记小鼠肿瘤模型中的新生血管内皮细胞，为抗血管生成治疗提供检测工具。
3. **文献名称**: "Comparative analysis of CDH5 antibody performance in immunohistochemical staining"
**作者**: Garcia R et al.
**摘要**: 对比了5种市售CDH5抗体在人类组织切片中的染色效果，发现抗体#ab123(兔多克隆)在福尔马林固定石蜡包埋样本中具有最优的灵敏度和背景信噪比，适用于病理诊断。
4. **文献名称**: "CDH5-targeted antibody-drug conjugate inhibits metastatic dissemination in vivo"
**作者**: Watanabe K et al.
**摘要**: 利用CDH5抗体偶联化疗药物，在肺癌小鼠模型中显示特异性靶向肿瘤血管系统，显著降低远处转移率，提示CDH5抗体在靶向治疗中的潜在应用。
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**注意**：以上为模拟内容，实际文献请通过PubMed/Google Scholar检索关键词如"CDH5 antibody validation"、"VE-cadherin antibody application"等。
CDH5. also known as VE-cadherin (vascular endothelial cadherin), is a calcium-dependent transmembrane glycoprotein belonging to the classical cadherin family. It plays a critical role in endothelial cell-cell adhesion, maintaining vascular integrity, and regulating vascular permeability. Primarily expressed in vascular endothelial cells, CDH5 mediates homotypic interactions through its extracellular domains, while its cytoplasmic tail interacts with catenins (e.g., β-catenin) to link the actin cytoskeleton, ensuring stable endothelial junctions.
CDH5 antibodies are essential tools for studying vascular biology, angiogenesis, and endothelial dysfunction in diseases like cancer, atherosclerosis, and inflammatory disorders. They enable the detection, localization, and functional analysis of CDH5 in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. Researchers use these antibodies to investigate endothelial barrier regulation, leukocyte transmigration, and tumor angiogenesis, as CDH5 dysregulation is associated with metastasis and pathological vascular remodeling.
Commercially available CDH5 antibodies are typically raised in rabbits or mice, targeting specific epitopes (e.g., extracellular or intracellular domains). Validation includes knockout controls or siRNA-mediated knockdown to ensure specificity. Their applications extend to preclinical models, aiding drug development targeting vascular leakage or anti-angiogenic therapies.
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