| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ARTS; DFN2; PRSI; CMTX5; DFNX1; PRS-I; PPRibP/PRSII/PRPS1; PRPS3; PRPSL; PRS-III |
| Entrez GeneID | 5631; |
| WB Predicted band size | 35kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthetic peptide corresponding to residues near the N terminal of human phosphoribosyl pyrophosphate synthetase 1/2/1-like 1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PRPS1、PRPS2和PRPS1L1抗体的3篇代表性文献摘要(注：文献为示例性概括，非真实存在)：
1. **"PRPS1 mutations drive resistance to chemotherapy in leukemia by altering nucleotide metabolism"**
- 作者：Zhang Y, et al.
- 摘要：研究揭示了PRPS1基因突变通过增强嘌呤合成通路导致急性白血病化疗耐药，研究中采用特异性PRPS1抗体检测患者样本中突变蛋白的表达水平及亚细胞定位。
2. **"Characterization of PRPS2 in hepatocellular carcinoma progression using novel monoclonal antibodies"**
- 作者：Li H, et al.
- 摘要：开发了高特异性PRPS2单克隆抗体，证实PRPS2在肝癌组织中异常高表达，并通过抗体介导的蛋白功能抑制实验证明其促进肿瘤细胞增殖的机制。
3. **"PRPS1L1 regulates neurodevelopment via purine biosynthesis: Insights from knockout mouse models"**
- 作者：Wang X, et al.
- 摘要：利用PRPS1L1抗体在基因敲除小鼠模型中验证蛋白表达缺失，发现PRPS1L1缺失导致神经管发育缺陷，提示其在嘌呤代谢相关神经疾病中的潜在作用。
(注：以上为基于领域知识的模拟文献，实际文献需通过PubMed/Google Scholar等平台检索确认。)
PRPS1. PRPS2. and PRPS1L1 are isoforms of phosphoribosyl pyrophosphate synthetase (PRPS), a key enzyme in nucleotide metabolism that catalyzes the synthesis of phosphoribosyl pyrophosphate (PRPP). PRPP serves as a critical precursor for purine, pyrimidine, and cofactor biosynthesis. PRPS1 and PRPS2 are catalytically active paralogs, with PRPS1 being ubiquitously expressed and PRPS2 showing higher expression in proliferative tissues. PRPS1L1 (PRS-A1) is a retrotransposed, intronless variant of PRPS1 but lacks enzymatic activity due to structural modifications, potentially acting as a regulatory element.
Antibodies targeting PRPS1/2/1L1 are essential tools for studying their expression, localization, and functional roles in health and disease. Dysregulation of PRPS isoforms is linked to various disorders. PRPS1 mutations are associated with inherited conditions like Arts syndrome (neuropathy and hearing loss) and Charcot-Marie-Tooth disease. PRPS2 overexpression has been implicated in cancer progression, including leukemia and solid tumors, likely due to increased nucleotide demand in proliferating cells. PRPS1L1’s role remains less defined, though it may modulate PRPS1/2 activity or interact with signaling pathways.
These antibodies enable researchers to investigate isoform-specific contributions to metabolic reprogramming, therapeutic resistance, or genetic disorders via techniques like Western blotting, immunohistochemistry, or functional assays. Their development supports precision in distinguishing between highly homologous isoforms, aiding mechanistic insights into nucleotide metabolism-related pathologies.
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