WB | 咨询技术 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 咨询技术 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | TCF-1 |
Entrez GeneID | 6932; |
WB Predicted band size | 42kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human,Mouse |
Immunogen | Synthetic peptide corresponding to a region derived from internal residues of human transcription factor 7 (T-cell specific, HMG-box) |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于TCF7抗体的3篇参考文献示例,包含文献名称、作者及摘要内容概括:
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1. **文献名称**:TCF7 regulates the CD8⁺ T cell response to acute infection
**作者**:Zhou X, Yu S, Zhao DM, et al.
**摘要**:该研究通过Western blot和流式细胞术利用TCF7抗体,揭示TCF7在CD8⁺ T细胞抗病毒免疫应答中的关键作用,证明其缺失导致记忆T细胞分化受损。
2. **文献名称**:TCF7-dependent Wnt signaling controls thymic senescence
**作者**:Hossain M, Qadri SM, Liu L.
**摘要**:通过免疫组织化学和染色质免疫沉淀(ChIP)结合TCF7抗体,发现TCF7介导的Wnt通路调控胸腺上皮细胞衰老,影响T细胞发育稳态。
3. **文献名称**:TCF7 expression in colorectal cancer correlates with stem-like phenotypes
**作者**:Chen J, Wang Y, Zhang Y, et al.
**摘要**:研究采用TCF7抗体进行免疫荧光和免疫组化分析,证实TCF7高表达与结直肠癌干细胞特性及化疗耐药性显著相关,提示其作为潜在治疗靶点。
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以上文献均以TCF7抗体为核心实验工具,涵盖免疫学、发育生物学及肿瘤学领域的研究应用。
**Background of TCF7 Antibody**
TCF7 (T-cell factor 7), also known as TCF-1. is a transcription factor encoded by the *TCF7* gene and belongs to the TCF/LEF (T-cell factor/lymphoid enhancer-binding factor) family. These proteins are key mediators of the Wnt/β-catenin signaling pathway, which regulates cell proliferation, differentiation, and stem cell maintenance. TCF7 contains a high-mobility group (HMG) DNA-binding domain that enables sequence-specific interactions with target genes, and it often partners with β-catenin to activate Wnt-responsive genes.
In the immune system, TCF7 is critical for T-cell development and lineage commitment in the thymus. It promotes the differentiation of naïve T cells into memory or effector subsets and sustains stem-like properties in progenitor T cells. Dysregulation of TCF7 is implicated in cancers, particularly T-cell acute lymphoblastic leukemia (T-ALL), where it may act as an oncogene or tumor suppressor depending on context.
Antibodies targeting TCF7 are essential tools for studying its expression, localization, and function. They are widely used in techniques like Western blotting, immunofluorescence, chromatin immunoprecipitation (ChIP), and flow cytometry to investigate TCF7's role in Wnt signaling, immune regulation, and cancer biology. Such antibodies help identify TCF7 as a biomarker or therapeutic target in diseases linked to Wnt pathway dysregulation.
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