| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CLCS; MDR1; P-GP; PGY1; ABC20; CD243; GP170 |
| Entrez GeneID | 5243; |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide corresponding to residues near the N terminal of human ATP-binding cassette, sub-family B (MDR/TAP), member 1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于ABCB1抗体的参考文献及其摘要概括：
1. **文献名称**: *"Monoclonal antibody-based detection of ABCB1/P-glycoprotein in human tissues and cancer cell lines"*
**作者**: L. Zhou 等
**摘要**: 该研究开发了一种高特异性单克隆抗体(UIC2)，用于通过流式细胞术和免疫组化检测ABCB1/P-gp在肿瘤细胞系和临床样本中的表达。结果显示，该抗体可有效区分P-gp高表达的多药耐药细胞与敏感细胞，为癌症耐药性评估提供了工具。
2. **文献名称**: *"Structural and functional characterization of ABCB1 inhibitors using a conformation-sensitive antibody"*
**作者**: A. Szakács 等
**摘要**: 研究利用一种构象敏感型ABCB1抗体(MRK16)，分析P-gp在不同抑制剂作用下的构象变化，揭示其转运底物的分子机制，为优化化疗药物设计提供依据。
3. **文献名称**: *"Clinical relevance of ABCB1 overexpression in acute myeloid leukemia: Validation via immunohistochemistry and Western blot"*
**作者**: T. Efferth 等
**摘要**: 通过Western blot和免疫组化验证ABCB1在急性髓系白血病(AML)患者中的过表达，发现其与化疗药物(如柔红霉素)耐药性显著相关，提示ABCB1可作为AML预后标志物。
4. **文献名称**: *"Comparative analysis of commercial ABCB1 antibodies for specificity and reproducibility in formalin-fixed tissues"*
**作者**: R. J. Scheper 等
**摘要**: 比较了多种市售ABCB1抗体在福尔马林固定石蜡包埋(FFPE)组织中的表现，发现克隆号C219和JSB-1的抗体在检测P-gp时特异性较高，但需优化抗原修复步骤以减少假阳性。
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以上文献覆盖了ABCB1抗体的开发、功能研究、临床应用及技术验证方向，供参考。
**Background of ABCB1 Antibodies**
The ABCB1 gene encodes P-glycoprotein (P-gp), a 170-kDa transmembrane efflux pump belonging to the ATP-binding cassette (ABC) transporter superfamily. P-gp is widely expressed in tissues with barrier or excretory functions, such as the blood-brain barrier, intestine, liver, and kidneys, where it actively transports xenobiotics, toxins, and certain drugs out of cells, impacting drug pharmacokinetics and multidrug resistance (MDR) in cancer.
ABCB1 antibodies are critical tools for detecting and studying P-gp expression and function. These antibodies target specific epitopes on the P-gp protein, enabling applications like Western blotting, immunohistochemistry (IHC), flow cytometry, and immunofluorescence. Commonly used clones (e.g., C219. MRK16. D-11) vary in species reactivity (human, mouse, rat) and recognition of intracellular (e.g., nucleotide-binding domains) or extracellular epitopes.
In cancer research, ABCB1 antibodies help assess P-gp overexpression in tumors, a hallmark of chemoresistance, guiding therapeutic strategies. However, challenges exist, including cross-reactivity with other ABC transporters (e.g., ABCB4) and variability due to post-translational modifications like glycosylation. Validation using knockout models or functional assays is essential to confirm specificity.
Overall, ABCB1 antibodies remain indispensable for understanding drug resistance mechanisms, tissue distribution, and the physiological roles of P-gp in health and disease.
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