| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MC3; OB20; OQTL; BMIQ9; MC3-R |
| Entrez GeneID | 4159; |
| WB Predicted band size | 36kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | Synthetic peptide corresponding to residues near the N terminal of human melanocortin 3 receptor |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MC3R抗体的3篇参考文献示例(注：以下内容为模拟文献，实际引用请核实原文)：
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1. **文献名称**：*Immunolocalization of Melanocortin-3 Receptor (MC3R) in the Hypothalamic Feeding Circuits*
**作者**：Smith A., Doe J., Cone R.D.
**摘要**：该研究通过免疫组化技术，利用特异性MC3R抗体标记了大鼠下丘脑中的神经元分布，发现MC3R在弓状核和室旁核高表达，提示其在调节摄食行为和能量代谢中的关键作用。
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2. **文献名称**：*MC3R Antibody-Based Analysis of Receptor Expression in Obesity Models*
**作者**：Chen L., Williams K.W., Elmquist J.K.
**摘要**：研究使用MC3R抗体进行Western blot和免疫荧光分析，对比了肥胖小鼠与正常小鼠下丘脑组织中MC3R的蛋白表达差异，发现肥胖模型中MC3R表达下调可能与代谢失调相关。
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3. **文献名称**：*Functional Characterization of MC3R Antibody in Knockout Mouse Studies*
**作者**：Butler A.A., Marks D.L., Fan W.
**摘要**：通过基因敲除小鼠模型结合MC3R抗体验证，揭示了MC3R缺失导致能量平衡异常和脂肪积累增加的机制，抗体特异性在受体功能研究中得到验证。
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(如需更多文献，建议通过PubMed或Google Scholar以“MC3R antibody”或“Melanocortin-3 Receptor localization”等关键词检索。)
The melanocortin-3 receptor (MC3R) is a G protein-coupled receptor (GPCR) primarily expressed in the central nervous system, particularly in hypothalamic regions involved in energy homeostasis. It binds α-melanocyte-stimulating hormone (α-MSH) and other melanocortin peptides, playing a key role in regulating appetite, energy expenditure, and metabolic adaptation. Unlike MC4R, which is strongly linked to obesity, MC3R is implicated in nutrient partitioning, circadian metabolism, and the adaptation to fasting. Dysregulation of MC3R signaling has been associated with metabolic disorders, including obesity and insulin resistance.
MC3R antibodies are essential tools for studying receptor localization, expression patterns, and signaling mechanisms. They enable the detection of MC3R in tissues via immunohistochemistry (IHC), Western blotting, or flow cytometry, aiding research into its physiological roles. Specific monoclonal or polyclonal antibodies help investigate receptor-ligand interactions, post-translational modifications, and downstream pathways. Recent studies also explore MC3R antibodies as potential therapeutics to modulate receptor activity in metabolic diseases. However, challenges remain in ensuring antibody specificity due to structural similarities among melanocortin receptors. Validation via knockout controls or functional assays is critical for reliable data. Overall, MC3R antibodies are pivotal in unraveling the receptor's complex biology and translational potential.
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