| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ASK1; MEKK5; MAPKKK5 |
| Entrez GeneID | 4217; |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide corresponding to residues near the N terminal of human mitogen-activated protein kinase kinase kinase 5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MAP3K5抗体的3篇参考文献示例(注：内容为虚构，仅供参考格式)：
1. **文献名称**：*MAP3K5 regulates apoptosis in hepatocellular carcinoma via JNK signaling*
**作者**：Chen L, Wang Y, et al.
**摘要**：本研究通过Western blot和免疫组化分析，发现MAP3K5在肝癌组织中高表达，其抗体特异性验证显示其通过激活JNK通路促进肿瘤细胞凋亡。
2. **文献名称**：*Role of MAP3K5 in neuroinflammation and Alzheimer’s disease pathology*
**作者**：Smith J, Patel R, et al.
**摘要**：利用MAP3K5抗体进行脑组织免疫荧光染色，揭示MAP3K5在小胶质细胞中的激活与Tau蛋白异常磷酸化相关，提示其在神经退行性疾病中的调控作用。
3. **文献名称**：*Validation of a novel MAP3K5 monoclonal antibody for flow cytometry applications*
**作者**：Zhang H, Kimura T, et al.
**摘要**：该文献报道了一种高特异性MAP3K5单克隆抗体的开发与验证，证明其在流式细胞术和免疫沉淀实验中可有效检测内源性MAP3K5蛋白表达。
(示例文献仅供格式参考，实际文献需通过学术数据库检索获取。)
The MAP3K5 antibody targets Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5), also known as apoptosis signal-regulating kinase 1 (ASK1), a serine/threonine kinase in the MAPK signaling pathway. MAP3K5 plays a critical role in cellular responses to stress, inflammation, apoptosis, and differentiation by activating downstream kinases like JNK and p38. It is activated by oxidative stress, cytokines, and endoplasmic reticulum stress, making it a key mediator in pathologies such as cancer, neurodegenerative diseases, and cardiovascular disorders. Dysregulation of MAP3K5 is linked to tumor progression, fibrosis, and metabolic syndromes, highlighting its therapeutic potential.
MAP3K5 antibodies are essential tools for studying its expression, activation, and interactions in research. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect protein levels, phosphorylation status (e.g., Thr845/Thr838), or localization in tissues and cell lines. These antibodies help elucidate MAP3K5's role in signaling cascades, drug response studies, and disease mechanisms. Many are validated for specificity using knockout controls or peptide blocking assays. Both monoclonal and polyclonal variants exist, with some designed to distinguish phosphorylated (active) from inactive forms. Researchers also employ MAP3K5 antibodies in functional assays to explore its involvement in apoptosis or inflammation, aiding the development of targeted therapies against MAP3K5-driven diseases.
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