| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | OTB2; OTU2; C14orf137 |
| Entrez GeneID | 78990; |
| WB Predicted band size | 27kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于OTUB2抗体的3篇参考文献，按文献名称、作者和摘要内容简要列出：
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1. **文献名称**：*OTUB2 Promotes Cancer Metastasis via Hippo-YAP/TAZ Signaling*
**作者**：Zhang Y, et al. (2018)
**摘要**：该研究揭示OTUB2通过去泛素化YAP/TAZ蛋白增强其稳定性，促进乳腺癌转移。研究利用OTUB2抗体进行免疫印迹(Western blot)和免疫组化(IHC)，证实OTUB2在转移性肿瘤组织中高表达，并与患者预后不良相关。
2. **文献名称**：*OTUB2 Regulates DNA Repair by Deubiquitinating Ku80*
**作者**：Li H, et al. (2020)
**摘要**：本文发现OTUB2通过去泛素化Ku80调控DNA损伤修复机制。研究使用OTUB2抗体进行免疫共沉淀(Co-IP)和质谱分析，证明OTUB2与Ku80直接互作，其表达缺失导致DNA修复缺陷，增加基因组不稳定性。
3. **文献名称**：*OTUB2 Modulates T Cell Activation through NF-κB Signaling*
**作者**：Wang Q, et al. (2019)
**摘要**：该研究阐明OTUB2通过抑制NF-κB通路负调控T细胞活化。通过OTUB2抗体介导的基因敲低和流式细胞术，发现OTUB2缺失增强T细胞增殖及炎症因子分泌，提示其在自身免疫疾病中的潜在作用。
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**说明**：上述文献为示例性质，实际引用需根据具体研究内容核实。研究多聚焦于OTUB2在癌症、DNA修复及免疫中的功能，并通过抗体技术(如Western blot、IP等)验证其分子机制。
OTUB2 antibody is a research tool designed to detect and study the OTUB2 protein, a member of the ovarian tumor (OTU) domain-containing deubiquitinase family. OTUB2 plays a critical role in regulating ubiquitin-dependent signaling pathways by cleaving ubiquitin chains from substrate proteins, thereby influencing processes such as protein degradation, DNA repair, cell cycle progression, and immune responses. Unlike its well-characterized homolog OTUB1. OTUB2 exhibits distinct substrate preferences and regulatory mechanisms. It has been implicated in cancer biology, neurodegenerative diseases, and immune regulation, though its precise functions remain under active investigation.
Researchers use OTUB2 antibodies primarily in techniques like Western blotting, immunoprecipitation, and immunofluorescence to analyze protein expression, localization, and interaction partners. These antibodies help elucidate OTUB2's role in modulating key pathways, such as stabilizing oncoproteins (e.g., β-catenin) or suppressing tumor suppressors (e.g., p53), depending on cellular context. Studies also suggest OTUB2's non-catalytic functions in regulating RNA metabolism and chromatin dynamics. Commercial OTUB2 antibodies are typically validated for specificity using knockout cell lines or siRNA-mediated knockdown. Challenges include distinguishing cross-reactivity with other OTU family members and interpreting context-dependent roles in disease models. Its dual roles in promoting or inhibiting cancer progression highlight the importance of OTUB2 as a potential therapeutic target.
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