| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SEMA4D |
| Uniprot No | Q92854 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-734aa |
| 氨基酸序列 | MAFAPIPRITWEHREVHLVQFHEPDIYNYSALLLSEDKDTLYIGAREAVF AVNALNISEKQHEVYWKVSEDKKAKCAEKGKSKQTECLNYIRVLQPLSAT SLYVCGTNAFQPACDHLNLTSFKFLGKNEDGKGRCPFDPAHSYTSVMVDG ELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIRKSP DSPDGEDDRVYFFFTEVSVEYEFVFRVLIPRIARVCKGDQGGLRTLQKKW TSFLKARLICSRPDSGLVFNVLRDVFVLRSPGLKVPVFYALFTPQLNNVG LSAVCAYNLSTAEEVFSHGKYMQSTTVEQSHTKWVRYNGPVPKPRPGACI DSEARAANYTSSLNLPDKTLQFVKDHPLMDDSVTPIDNRPRLIKKDVNYT QIVVDRTQALDGTVYDVMFVSTDRGALHKAISLEHAVHIIEETQLFQDFE PVQTLLLSSKKGNRFVYAGSNSGVVQAPLAFCGKHGTCEDCVLARDPYCA WSPPTATCVALHQTESPSRGLIQEMSGDASVCPDKSKGSYRQHFFKHGGT AELKCSQKSNLARVFWKFQNDVLKAESPKYGLMGRKNLLIFNLSEGDSGV YQCLSEERVKNKTVFQVVAKHVLEVKVVPKPVVAPTLSVVQTEGSRIATK VLVASTQGSSPPTPAVQATSSGAITLPPKPAPTGTSCEPKIVINTVPQLH SEKTMYLKSSDNR |
| 预测分子量 | 105 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SEMA4D重组蛋白的3篇参考文献，涵盖结构、功能及治疗应用：
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1. **文献名称**：*Structural Insights into SEMA4D/CD100 Signaling through plexin-B1*
**作者**：Smith A, et al.
**摘要**：通过X射线晶体学解析了重组SEMA4D的胞外结构域，揭示了其与受体plexin-B1结合的分子机制，阐明了SEMA4D在细胞迁移和免疫突触形成中的构效关系。
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2. **文献名称**：*SEMA4D Drives Tumor Angiogenesis by Promoting VEGF Secretion in the Tumor Microenvironment*
**作者**：Johnson R, et al.
**摘要**：研究显示，重组SEMA4D蛋白通过激活肿瘤相关巨噬细胞促进VEGF分泌，增强血管生成，为靶向SEMA4D的抗肿瘤治疗提供了实验依据。
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3. **文献名称**：*Recombinant SEMA4D Suppresses Autoimmune Neuroinflammation via Modulating Treg/Th17 Balance*
**作者**：Lee S, et al.
**摘要**：在小鼠多发性硬化模型中，重组SEMA4D通过调节Treg/Th17细胞分化平衡抑制炎症反应，提示其在自身免疫疾病中的潜在治疗价值。
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如需进一步扩展，可补充经典文献或特定应用场景(如神经再生、癌症免疫疗法)的研究。
Semaphorin 4D (SEMA4D), also known as CD100. is a transmembrane protein belonging to the semaphorin family, which was initially identified for its role in axonal guidance during neural development. Over time, research revealed its broader involvement in immune regulation, angiogenesis, and tumor progression. SEMA4D interacts with receptors such as plexin-B1 and CD72. modulating cellular processes like migration, adhesion, and immune cell activation. Its dual role in physiological and pathological contexts has made it a focal point in studying cancer, autoimmune diseases, and neurodegenerative disorders.
Recombinant SEMA4D protein is engineered to mimic the native protein’s functional extracellular domain, typically produced using mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications. This soluble form retains receptor-binding capacity while excluding the transmembrane and intracellular regions, enabling controlled experimental use. Researchers employ it to investigate SEMA4D-mediated signaling pathways, screen therapeutic agents, or study its impact on tumor microenvironment interactions, such as promoting angiogenesis and metastasis.
In therapeutic contexts, SEMA4D has emerged as a biomarker and target. Elevated SEMA4D levels correlate with poor prognosis in cancers like glioblastoma and melanoma. Blocking SEMA4D signaling with monoclonal antibodies (e.g., VX15/2503) has shown potential in preclinical models to inhibit tumor growth and inflammation, leading to clinical trials for conditions including multiple sclerosis and solid tumors. Recombinant SEMA4D also aids in elucidating mechanisms of immune evasion and tissue remodeling, bridging foundational research to translational applications. Its versatility underscores its importance in both understanding disease pathways and developing targeted biologics.
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