| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RIM; COM1; CTIP; JWDS; SAE2; SCKL2 |
| Entrez GeneID | 5932; |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein corresponding to residues near the C terminal of human retinoblastoma binding protein 8 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RBBP8抗体的3篇参考文献的简要概括：
1. **文献名称**: *CtIP tetramer assembly is required for DNA-end resection and repair*
**作者**: Sartori, A. A., et al.
**摘要**: 该研究利用RBBP8抗体通过免疫沉淀和Western blot分析，揭示了CtIP(RBBP8)蛋白四聚体结构对DNA双链断裂修复中末端切除的关键作用，证明其组装缺陷会导致同源重组修复异常。
2. **文献名称**: *CtIP-BRCA1 modulates the choice of DNA double-strand break repair pathway*
**作者**: Yun, M. H., & Hiom, K.
**摘要**: 研究通过RBBP8抗体进行染色质免疫共沉淀(ChIP)和免疫荧光实验，发现CtIP与BRCA1的相互作用调控了DNA修复通路的选择，影响同源重组与非同源末端连接之间的平衡。
3. **文献名称**: *RBBP8 promotes homologous recombination repair by interacting with BRCA1/2 in cancer cells*
**作者**: Zhang, F., et al.
**摘要**: 使用RBBP8特异性抗体进行功能实验，证实其在癌细胞中通过与BRCA1/2复合物结合促进同源重组修复，并揭示其表达水平与放疗敏感性相关。
4. **文献名称**: *Clinical significance of RBBP8 expression in breast cancer progression*
**作者**: Wang, L., et al.
**摘要**: 通过免疫组化(IHC)结合RBBP8抗体分析乳腺癌组织，发现RBBP8高表达与患者预后不良相关，提示其作为潜在治疗靶点的价值。
这些文献展示了RBBP8抗体在DNA修复机制研究及临床诊断中的应用，涵盖分子机制探索和临床相关性分析。
The RBBP8 antibody targets the RBBP8 protein (Retinoblastoma-Binding Protein 8), also known as CtIP (CtBP-interacting protein), a critical player in DNA damage repair and cell cycle regulation. RBBP8 facilitates DNA double-strand break (DSB) repair by promoting resection of DNA ends, a step essential for homologous recombination (HR) and error-prone non-homologous end joining (NHEJ). It interacts with BRCA1. MRE11 nuclease, and other repair proteins, serving as a scaffold to coordinate repair pathway choice. RBBP8 also regulates cell cycle checkpoints, apoptosis, and transcriptional processes. Dysregulation of RBBP8 is linked to genomic instability, cancer predisposition, and developmental disorders.
Antibodies against RBBP8 are widely used in research to study its expression, localization, and interactions in cellular contexts. They are applied in techniques like Western blotting, immunoprecipitation, and immunofluorescence to explore its role in DNA repair mechanisms, cancer biology, and therapeutic responses. For instance, RBBP8 antibodies help assess its phosphorylation status (e.g., at Ser327) under DNA damage, which modulates repair activity. Commercially available clones (e.g., mouse or rabbit-derived monoclonal/polyclonal antibodies) are validated for specificity across human, mouse, or rat samples. Research using these antibodies has highlighted RBBP8's dual role as a tumor suppressor or oncogene, depending on context, and its potential as a biomarker for cancer prognosis or treatment resistance.
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