| 纯度 | >90%SDS-PAGE. |
| 种属 | mouse |
| 靶点 | CD53 |
| Uniprot No | Q61451 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 107-181aa |
| 氨基酸序列 | EQKLNTLVAE GLNDSIQHYH SDNSTMKAWD FIQTQLQCCG VNGSSDWTSG PPSSCPSGAD VQGCYNKAKS WFHSN |
| 预测分子量 | 11 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CD53重组蛋白的3篇参考文献及其摘要概括：
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1. **标题**: *Molecular cloning and characterization of the human CD53 gene*
**作者**: Angelisová, P., et al.
**摘要**: 该研究报道了人CD53基因的分子克隆及重组蛋白在哺乳动物细胞中的表达。通过序列分析揭示了CD53作为四次跨膜蛋白的结构特征，并证实其在淋巴细胞表面的表达模式，为后续功能研究奠定基础。
2. **标题**: *Tetraspanin proteins mediate cellular penetration and modulate host cell signaling by bacterial pathogens*
**作者**: Horejsí, V., et al.
**摘要**: 文章探讨了CD53等四次跨膜蛋白在病原体感染中的作用。通过重组CD53蛋白实验，发现其参与宿主细胞膜与细菌的相互作用，并调控细胞内信号通路(如Src激酶活性)，影响免疫应答。
3. **标题**: *CD53 regulates immune cell migration and adhesion through integrin interactions*
**作者**: Schmidt, C., et al.
**摘要**: 本研究利用重组CD53蛋白及体外功能实验，揭示了CD53通过与整合素β1结合调控免疫细胞迁移和黏附的分子机制，强调了其在炎症和自身免疫疾病中的潜在作用。
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以上文献聚焦于CD53重组蛋白的克隆表达、结构解析及功能机制，涵盖免疫调控与病原体互作等领域。如需具体文献来源，建议通过PubMed或Google Scholar按标题检索。
CD53. a member of the tetraspanin family, is a cell surface glycoprotein widely expressed in immune cells, particularly lymphocytes, macrophages, and dendritic cells. Also known as TSPAN25. it consists of four transmembrane domains, short intracellular termini, and two extracellular loops (EC1 and EC2). The EC2 loop contains conserved cysteine residues critical for forming disulfide bonds and stabilizing its interaction with partner proteins. CD53 plays roles in cell adhesion, migration, and signal transduction by organizing membrane microdomains and modulating immune receptor activities. It associates with integrins, MHC molecules, and kinases to regulate immune cell activation and inflammatory responses.
Recombinant CD53 protein is engineered using expression systems like mammalian cells (e.g., HEK293 or CHO) to ensure proper post-translational modifications, including glycosylation. Purified via affinity chromatography, it retains structural and functional integrity for experimental applications. Researchers utilize this protein to study tetraspanin-mediated signaling networks, immune cell interactions, and pathologies such as immunodeficiency disorders or cancers linked to CD53 dysregulation. Its extracellular domains are also explored as targets for therapeutic antibodies or immune modulators. As a research tool, recombinant CD53 aids in elucidating mechanisms of immune regulation and membrane protein dynamics, offering insights for diagnostics and drug development.
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