| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TIMP1 |
| Uniprot No | P01033 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-207aa |
| 氨基酸序列 | CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQ ALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLQGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIA |
| 预测分子量 | 21 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TIMP1重组蛋白的3-4条参考文献示例(注：文献信息为假设性概括，需根据实际检索调整)：
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1. **文献名称**：*Expression and Functional Characterization of Recombinant Human TIMP-1 in Mammalian Cells*
**作者**：Smith J, et al.
**摘要**：研究通过哺乳动物表达系统成功表达并纯化具有生物活性的重组人TIMP1蛋白，验证其抑制基质金属蛋白酶(MMP-9)的能力，并证明其在体外抑制肿瘤细胞侵袭的作用。
2. **文献名称**：*Recombinant TIMP-1 Attenuates Cardiac Fibrosis via MMP Inhibition in a Murine Model*
**作者**：Li Y, et al.
**摘要**：在小鼠心肌纤维化模型中，重组TIMP1蛋白通过抑制MMP活性减少胶原沉积，改善心脏功能，提示其在抗纤维化治疗中的潜在应用。
3. **文献名称**：*Glycosylation Modulates the Stability and Activity of Recombinant TIMP-1 in Cancer Microenvironments*
**作者**：Garcia R, et al.
**摘要**：探讨了糖基化修饰对重组TIMP1蛋白稳定性和功能的影响，发现糖基化版本在肿瘤微环境中表现出更强的MMP抑制活性及抗血管生成作用。
4. **文献名称**：*Bacterial Expression of TIMP-1: Challenges and Solutions for Active Protein Yield*
**作者**：Wang H, et al.
**摘要**：分析大肠杆菌表达重组TIMP1的难点(如包涵体形成)，通过优化密码子、融合标签和复性策略提高可溶性活性蛋白的产量。
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如需具体文献，建议在PubMed或Google Scholar检索关键词：**"recombinant TIMP1" + expression/therapeutic application/MMP inhibition**。
**Background of TIMP1 Recombinant Protein**
Tissue Inhibitor of Metalloproteinases 1 (TIMP1) is a secreted glycoprotein belonging to the TIMP family, which comprises four members (TIMP1-4) that regulate extracellular matrix (ECM) homeostasis by inhibiting matrix metalloproteinases (MMPs). TIMP1 specifically targets MMP-9 (gelatinase B) and other MMPs, balancing proteolytic activity to prevent excessive ECM degradation. Beyond its canonical role in MMP inhibition, TIMP1 exhibits multifaceted functions, including cell growth promotion, apoptosis regulation, and modulation of signaling pathways via interactions with cell surface receptors like CD63 or integrins.
Recombinant TIMP1 protein is engineered using expression systems such as *E. coli* or mammalian cells, ensuring high purity and bioactivity for research and therapeutic applications. Its production enables precise study of TIMP1's dual roles in physiological and pathological contexts. For instance, TIMP1 is implicated in tissue repair, fibrosis, and cancer progression. While it suppresses tumor metastasis by inhibiting MMP-mediated ECM remodeling, paradoxically, elevated TIMP1 levels in some cancers correlate with poor prognosis, suggesting context-dependent pro-tumorigenic effects.
In research, recombinant TIMP1 is utilized to explore mechanisms underlying diseases like liver fibrosis, osteoarthritis, and neurodegenerative disorders. It also serves as a potential therapeutic candidate or biomarker. However, its pleiotropic nature complicates clinical translation, necessitating further studies to unravel cell-specific signaling pathways and therapeutic thresholds. Overall, TIMP1 recombinant protein remains a vital tool for deciphering ECM biology and developing targeted therapies for MMP-related pathologies.
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