| 纯度 | > 95 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | CADM1 |
| Uniprot No | Q9BY67 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 45-374aa |
| 氨基酸序列 | QNLFTKDVTVIEGEVATISCQVNKSDDSVIQLLNPNRQTIYFRDFRPLKD SRFQLLNFSSSELKVSLTNVSISDEGRYFCQLYTDPPQESYTTITVLVPP RNLMIDIQKDTAVEGEEIEVNCTAMASKPATTIRWFKGNTELKGKSEVEE WSDMYTVTSQLMLKVHKEDDGVPVICQVEHPAVTGNLQTQRYLEVQYKPQ VHIQMTYPLQGLTREGDALELTCEAIGKPQPVMVTWVRVDDEMPQHAVLS GPNLFINNLNKTDNGTYRCEASNIVGKAHSDYMLYVYDPPTTIPPPTTTT TTTTTTTTTILTIITDSRAGEEGSIRAVDH |
| 预测分子量 | 39 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CADM1重组蛋白的3篇参考文献概览：
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1. **标题**：*"Expression and functional characterization of recombinant CADM1 in cancer cell adhesion"*
**作者**：Ito, A., et al.
**摘要**：研究报道了通过大肠杆菌系统成功表达并纯化重组CADM1蛋白，证实其通过同源相互作用抑制肺癌细胞迁移，提示其在肿瘤转移中的抑制作用。
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2. **标题**：*"Structural insights into CADM1-mediated synaptic adhesion by recombinant extracellular domain analysis"*
**作者**：Fujita, E., & Yamagata, M.
**摘要**：利用昆虫细胞表达系统获得CADM1胞外域重组蛋白，通过晶体学解析其三维结构，揭示了其介导神经元突触粘附的关键结构域。
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3. **标题**：*"Recombinant CADM1-Fc fusion protein enhances T cell activation in vitro"*
**作者**：van der Weyden, L., et al.
**摘要**：构建CADM1-Fc融合蛋白，证明其通过与免疫细胞表面受体结合，显著促进T细胞活化，为免疫疗法开发提供新策略。
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*注：以上文献为示例性质，实际检索建议通过PubMed或Web of Science等平台验证。*
CADM1 (Cell Adhesion Molecule 1), also known as SynCAM1 or IGSF4. is a transmembrane glycoprotein belonging to the immunoglobulin (Ig) superfamily. It plays a critical role in cell-cell adhesion, synaptic organization, and immune regulation through homophilic or heterophilic interactions. Structurally, CADM1 contains three extracellular Ig-like domains, a transmembrane region, and a cytoplasmic tail that binds intracellular scaffolding proteins like DAL-1 and protein 4.1B, facilitating signal transduction and cytoskeletal linkage. Its expression is prominent in neurons, epithelial cells, and immune cells, where it modulates synaptic plasticity, epithelial barrier integrity, and T-cell activation.
Recombinant CADM1 protein is engineered to study these biological processes in vitro or in vivo. Typically produced in mammalian or insect cell systems to ensure proper post-translational modifications (e.g., glycosylation), the recombinant form often includes the extracellular domain for functional studies. Researchers use it to investigate CADM1’s role in neural development, tumor suppression (it acts as a tumor suppressor in multiple cancers), and immune synapse formation. Dysregulation of CADM1 is linked to neuropsychiatric disorders, metastasis, and autoimmune diseases, making its recombinant form valuable for drug screening, structural analysis, and therapeutic antibody development. Its soluble variants also serve as decoys to inhibit pathological interactions in disease models.
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