| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDOST |
| Uniprot No | P39656 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-456aa |
| 氨基酸序列 | MGYFRCAGAGSFGRRRKMEPSTAARAWALFWLLLPLLGAVCASGPRTLVL LDNLNVRETHSLFFRSLKDRGFELTFKTADDPSLSLIKYGEFLYDNLIIF SPSVEDFGGNINVETISAFIDGGGSVLVAASSDIGDPLRELGSECGIEFD EEKTAVIDHHNYDISDLGQHTLIVADTENLLKAPTIVGKSSLNPILFRGV GMVADPDNPLVLDILTGSSTSYSFFPDKPITQYPHAVGKNTLLIAGLQAR NNARVIFSGSLDFFSDSFFNSAVQKAAPGSQRYSQTGNYELAVALSRWVF KEEGVLRVGPVSHHRVGETAPPNAYTVTDLVEYSIVIQQLSNGKWVPFDG DDIQLEFVRIDPFVRTFLKKKGGKYSVQFKLPDVYGVFQFKVDYNRLGYT HLYSSTQVSVRPLQHTQYERFIPSAYPYYASAFSMMLGLFIFSIVFLHMK EKEKSD |
| 预测分子量 | 77 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DDOST重组蛋白的示例参考文献(仅供参考，建议通过学术数据库查询真实文献)：
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1. **标题**: "Expression and Purification of Recombinant DDOST Protein in Escherichia coli"
**作者**: Zhang L, et al.
**摘要**: 研究报道了在大肠杆菌系统中高效表达重组DDOST蛋白的优化方法，通过亲和层析纯化获得高纯度蛋白，并验证其参与N-糖基化底物结合的酶活性。
2. **标题**: "Functional Characterization of Recombinant DDOST in Glycosylation-Deficient Cells"
**作者**: Kim S, Lee JH.
**摘要**: 利用重组DDOST蛋白在糖基化缺陷细胞中恢复蛋白质N-糖基化功能，证实其在内质网质量控制中的关键作用及与先天性糖基化疾病的相关性。
3. **标题**: "Structural Insights into DDOST-Mediated Oligosaccharyltransferase Complex Assembly"
**作者**: Wang Y, et al.
**摘要**: 通过冷冻电镜解析重组DDOST蛋白与OST复合物的三维结构，揭示其作为亚基在催化糖基化反应中的构象变化和分子互作机制。
4. **标题**: "Recombinant DDOST as a Novel Biomarker for Autoimmune Disorders"
**作者**: Patel R, et al.
**摘要**: 探讨重组DDOST蛋白在血清中的自身抗体检测价值，发现其在系统性红斑狼疮等自身免疫性疾病中的潜在诊断应用。
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注：上述文献信息为示例，实际研究请通过PubMed/Google Scholar等平台以“DDOST recombinant protein”为关键词检索。
**Background of DDOST Recombinant Protein**
DDOST (Dolichyl-diphosphooligosaccharide—protein glycosyltransferase), also known as RPN1 or OST48. is a key enzyme involved in N-linked protein glycosylation, a critical post-translational modification process. It functions as a subunit of the oligosaccharyltransferase (OST) complex within the endoplasmic reticulum (ER), catalyzing the transfer of preassembled oligosaccharides to specific asparagine residues on nascent polypeptides. This modification is essential for protein folding, stability, and cellular localization, impacting diverse biological processes, including immune response, cell adhesion, and signal transduction.
The recombinant DDOST protein is produced using genetic engineering techniques, typically by expressing the human *DDOST* gene in heterologous systems like *E. coli*, yeast, or mammalian cell lines. This allows large-scale production of the purified protein while retaining its functional properties. Recombinant DDOST serves as a valuable tool for studying N-glycosylation mechanisms, ER-associated degradation (ERAD), and diseases linked to glycosylation defects, such as congenital disorders of glycosylation (CDGs), cancer, and neurodegenerative conditions.
Research on DDOST also explores its role in modulating ER stress and unfolded protein response (UPR) pathways, which are implicated in metabolic and age-related diseases. Additionally, abnormal glycosylation patterns associated with DDOST dysfunction are investigated as potential biomarkers or therapeutic targets. The availability of recombinant DDOST protein facilitates structural studies, antibody development, and high-throughput screening for drug discovery, advancing both basic science and clinical applications in glycobiology and precision medicine.
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