| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CIS3; SSI3; ATOD4; Cish3; SSI-3; SOCS-3 |
| Entrez GeneID | 9021; |
| WB Predicted band size | 25kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于SOCS3抗体的模拟参考文献示例(非真实文献，仅供格式参考)：
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1. **文献名称**: *"SOCS3 regulates inflammation in macrophages by suppressing STAT3 activation"*
**作者**: Smith A, et al.
**摘要**: 研究利用SOCS3抗体通过Western blot和免疫荧光技术，证明SOCS3在巨噬细胞中高表达，并通过抑制STAT3磷酸化减轻内毒素诱导的炎症反应。
2. **文献名称**: *"SOCS3 expression in hepatocellular carcinoma: A prognostic biomarker study"*
**作者**: Li X, et al.
**摘要**: 通过免疫组化(使用SOCS3抗体)分析肝癌组织样本，发现SOCS3低表达与患者预后不良相关，提示其可能作为肿瘤抑制因子参与肝癌进展。
3. **文献名称**: *"SOCS3 modulates leptin resistance in obesity via JAK2/STAT3 signaling"*
**作者**: Johnson R, et al.
**摘要**: 研究在小鼠脂肪组织中使用SOCS3抗体检测蛋白水平，证实SOCS3过表达通过阻断瘦素信号通路导致肥胖相关的代谢紊乱。
4. **文献名称**: *"Role of SOCS3 in autoimmune encephalomyelitis: Insights from antibody-based inhibition"*
**作者**: Müller S, et al.
**摘要**: 通过SOCS3抗体阻断实验性自身免疫性脑脊髓炎(EAE)模型中的SOCS3功能，发现其缺失加剧神经炎症，提示SOCS3对自身免疫反应的保护作用。
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**注意**：以上为模拟示例，建议通过PubMed、Google Scholar等平台检索真实文献(关键词如“SOCS3 antibody application”“SOCS3 in disease”)。真实文献可能包括：
- **Nicholson et al. (1999)** 关于SOCS家族鉴定的经典研究。
- **Yasukawa et al. (2003)** 对SOCS3在信号通路中作用的深入分析。
The suppressor of cytokine signaling 3 (SOCS3) antibody is a critical tool in studying the SOCS3 protein, a member of the SOCS family that regulates cytokine signaling pathways. SOCS3 acts as a negative feedback inhibitor of the JAK-STAT signaling cascade, primarily by binding to cytokine receptors or JAK kinases to block STAT activation. It plays pivotal roles in immune regulation, inflammation, and cellular homeostasis, with dysregulation linked to diseases like cancer, autoimmune disorders, and metabolic syndromes.
SOCS3 antibodies are widely used in research to detect and quantify SOCS3 expression in tissues or cells via techniques such as Western blot, immunohistochemistry, and flow cytometry. These antibodies help elucidate SOCS3's involvement in pathological processes, including its tumor-suppressive or oncogenic roles in different cancers, its modulation of insulin resistance in diabetes, and its impact on inflammatory conditions like rheumatoid arthritis.
Developing high-specificity SOCS3 antibodies remains challenging due to structural similarities among SOCS family members. Researchers prioritize antibodies validated for cross-reactivity and target affinity to ensure experimental accuracy. As therapeutic strategies targeting SOCS3 emerge, such as in enhancing antiviral immunity or mitigating cytokine storms, reliable antibodies are essential for preclinical studies. Understanding SOCS3's dual regulatory functions through antibody-based assays continues to advance both basic and translational research in immunology and beyond.
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