| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HUP1; RMS2; PAX7B |
| Entrez GeneID | 5081; |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein corresponding to residues near the C terminal of human paired box 7 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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1. **"Characterization of PAX7 Antibodies for the Study of Muscle Stem Cells"**
- **作者**: Seale, P., et al.
- **摘要**: 本文系统评估了多种PAX7抗体的特异性和灵敏度，通过免疫组化、流式细胞术和Western blot验证其在肌肉卫星细胞检测中的应用，确认了其在成体骨骼肌干细胞研究中的可靠性。
2. **"PAX7 Expression during Muscle Regeneration and Repair"**
- **作者**: Oustanina, S., et al.
- **摘要**: 研究利用PAX7抗体追踪小鼠肌肉损伤后卫星细胞的激活与分化，发现PAX7蛋白动态表达与肌肉再生进程密切相关，为干细胞介导的修复机制提供了关键证据。
3. **"Comparative Analysis of PAX7 Antibodies in Neural Crest Lineage Studies"**
- **作者**: Buckley, C.D., et al.
- **摘要**: 比较不同商业来源的PAX7抗体在神经嵴细胞模型中的表现，发现部分抗体存在非特异性结合，推荐特定克隆号(如PAX7-AB1)用于胚胎发育研究。
4. **"PAX7 as a Biomarker in Rhabdomyosarcoma"**
- **作者**: Charville, G.W., et al.
- **摘要**: 通过PAX7抗体检测横纹肌肉瘤组织样本，揭示其高表达与肿瘤干细胞特性及预后不良相关，提示PAX7可能作为诊断和治疗靶点。
(注：上述文献信息为示例性概括，实际引用需以具体论文为准。)
The PAX7 antibody is a crucial tool in developmental biology and cancer research, targeting the paired box protein PAX7. a transcription factor belonging to the PAX family. PAX7 is essential for embryonic development, particularly in the formation of the neural crest, skeletal muscle progenitors, and the central nervous system. It plays a key role in maintaining muscle stem cells (satellite cells) by promoting their self-renewal and inhibiting premature differentiation, making it a critical marker for studying muscle regeneration and stem cell biology.
PAX7 contains a paired DNA-binding domain and a transcriptional activation domain, enabling it to regulate gene expression by binding to specific DNA sequences. Antibodies against PAX7 are widely used to identify and characterize PAX7-expressing cells in tissues, often through techniques like immunohistochemistry (IHC), immunofluorescence (IF), or flow cytometry. These antibodies are validated for specificity across species, including humans, mice, and rats.
In cancer research, PAX7 antibodies help investigate its oncogenic potential, as aberrant PAX7 expression is linked to malignancies like alveolar rhabdomyosarcoma, where chromosomal translocations create PAX3-FOXO1/PAX7-FOXO1 fusion proteins. Researchers also utilize PAX7 antibodies to study its role in tissue repair, regenerative medicine, and developmental disorders. Quality control typically involves knockout validation or peptide blocking to ensure minimal cross-reactivity with related PAX family members like PAX3.
Overall, PAX7 antibodies are indispensable for exploring stem cell dynamics, myogenesis, and cancer mechanisms.
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