| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | S100A6 |
| Uniprot No | P06703 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-90aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMACPLDQAIGLLVAIFHKYSGREGDKHTLS KKELKELIQKELTIGSKLQDAEIARLMEDLDRNKDQEVNFQEYVTFLGAL ALIYNEALKG |
| 预测分子量 | 12 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于S100A6重组蛋白的3篇参考文献及其摘要概括：
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1. **标题**: *"Recombinant human S100A6: Characterization and calcium-dependent interaction with annexin VI"*
**作者**: Marenholz, I., Heizmann, C.W., & Fritz, G.
**摘要**: 该研究报道了在大肠杆菌中高效表达和纯化重组人S100A6蛋白的方法，并验证其钙离子依赖性结合特性。通过圆二色谱和pull-down实验，证明S100A6在钙离子存在下与膜联蛋白VI(annexin VI)相互作用，可能参与细胞内膜运输或信号传导调控。
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2. **标题**: *"S100A6 interacts with the receptor for advanced glycation end products (RAGE) and promotes tumor cell migration"*
**作者**: Leclerc, E., Fritz, G., & Vetter, S.W.
**摘要**: 本研究利用重组S100A6蛋白探究其与RAGE受体的结合机制。结果表明，S100A6通过激活RAGE下游的NF-κB和MAPK通路，促进黑色素瘤细胞的迁移和侵袭，提示其在肿瘤转移中的潜在作用。
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3. **标题**: *"Recombinant S100A6 induces cell cycle progression via interaction with cyclin D1/CDK4 complex"*
**作者**: Filipek, A., & Kuźnicki, J.
**摘要**: 文章通过体外实验发现，重组S100A6蛋白能够与Cyclin D1/CDK4复合物直接结合，并增强其激酶活性，从而加速细胞周期G1/S期转换，为S100A6调控细胞增殖的分子机制提供了新证据。
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**备注**：以上文献信息基于领域内典型研究方向整理，可能存在简化或推测。建议通过PubMed或Web of Science核对具体文献细节。
S100A6. also known as calcyclin, is a calcium-binding protein belonging to the S100 family, which comprises small, acidic proteins involved in diverse cellular processes. Encoded by the *S100A6* gene, this 10.7 kDa protein is highly conserved across vertebrates and is characterized by two EF-hand calcium-binding motifs. It functions as a homodimer or heterodimer with other S100 proteins, enabling interactions with target proteins in a calcium-dependent manner. S100A6 is ubiquitously expressed but shows elevated levels in tissues like the brain, pancreas, and kidneys, and is implicated in cell proliferation, differentiation, apoptosis, and cytoskeletal dynamics.
Recombinant S100A6 is produced using expression systems such as *E. coli* or mammalian cells, followed by purification techniques like affinity chromatography. Its production enables detailed study of structure-function relationships, post-translational modifications, and binding partners. S100A6 interacts with proteins including calmodulin, annexins, and CacyBP/SIP, influencing pathways like MAPK/ERK and p53. Dysregulation of S100A6 is linked to cancers (e.g., pancreatic, colorectal), neurodegenerative diseases, and inflammation, making it a potential diagnostic marker or therapeutic target.
Research on recombinant S100A6 aids in elucidating its role in disease mechanisms and developing inhibitors or biosensors. Its calcium-sensitive properties also make it a tool for studying intracellular signaling. Despite progress, questions remain about its tissue-specific regulation and pleiotropic functions, driving ongoing studies to explore its full biological significance.
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