| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PEP-19 |
| Entrez GeneID | 5121; |
| WB Predicted band size | 7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Fusion protein corresponding to a region derived from internal residues of human purkinje cell protein 4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PCP4抗体的3篇参考文献示例(注：文献为虚拟示例，实际引用时请核实真实来源)：
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1. **文献名称**：*"Immunohistochemical analysis of PCP4 expression in cerebellar Purkinje cells during development"*
**作者**：Smith A, et al.
**摘要**：本研究利用特异性PCP4抗体，揭示了小鼠小脑浦肯野细胞中PCP4蛋白的发育依赖性表达模式，表明其可能通过调节钙离子信号通路参与神经元分化与突触可塑性。
2. **文献名称**：*"PCP4 as a potential biomarker in prostate cancer: Validation of a monoclonal antibody for clinical diagnostics"*
**作者**：Johnson B, et al.
**摘要**：通过开发高特异性PCP4单克隆抗体，研究证实PCP4在前列腺癌组织中显著高表达，且与肿瘤转移风险相关，提示其作为临床诊断标志物的潜力。
3. **文献名称**：*"Characterization of PCP4 knockout mice using antibody-based proteomic approaches"*
**作者**：Lee C, et al.
**摘要**：采用Western blot和免疫荧光技术，结合PCP4抗体系统分析基因敲除小鼠模型，发现PCP4缺失导致小脑功能异常，进一步阐明其在运动协调中的作用。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词“PCP4 antibody”、“PCP4 immunohistochemistry”等获取。
The Purkinje cell protein 4 (PCP4), also known as PEP-19. is a small, neuron-specific protein highly expressed in cerebellar Purkinje cells and select brain regions, including the hippocampus and thalamus. It regulates calcium-mediated signaling by interacting with calmodulin (CaM) through its IQ domain, modulating neuronal excitability, differentiation, and apoptosis. PCP4's role in calcium homeostasis and neurodevelopment has linked it to neurological disorders such as spinocerebellar ataxia and Alzheimer's disease. Dysregulation of PCP4 has also been observed in cancers, including breast and prostate cancer, suggesting potential oncogenic or tumor-suppressive functions depending on context.
PCP4 antibodies are essential tools for detecting and quantifying PCP4 expression in research applications like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies aid in studying PCP4's spatial and temporal expression patterns, its interaction with calcium-signaling pathways, and its involvement in disease mechanisms. Commercially available antibodies are typically raised in rabbits or mice, targeting specific epitopes within the protein's conserved regions. Validation often includes knockout controls to confirm specificity. Researchers utilize PCP4 antibodies to explore its dual roles in neuroprotection and pathology, as well as its potential as a diagnostic or therapeutic biomarker in neurological and oncological studies.
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