| 纯度 | >95%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | Smad5 |
| Uniprot No | P97454 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-465aa |
| 氨基酸序列 | TSMASLFSF TSPAVKRLLG WKQGDEEEKW AEKAVDALVK KLKKKKGAME ELEKALSSPG QPSKCVTIPR SLDGRLQVSH RKGLPHVIYC RVWRWPDLQS HHELKPLDIC EFPFGSKQKE VCINPYHYKR VESPVLPPVL VPRHNEFNPQ HSLLVQFRNL SHNEPHMPQN ATFPDSFHQP NNAPFPLSPN SPYPPSPASS TYPNSPASSG PGSPFQLPAD TPPPAYMPPD DQMAPDNSQP MDTSSNMIPQ TMPSISSRDV QPVAYEEPKH WCSIVYYELN NRVGEAFHAS STSVLVDGFT DPSNNKSRFC LGLLSNVNRN STIENTRRHI GKGVHLYYVG GEVYAECLSD SSIFVQSRNC NFHHGFHPTT VCKIPSSCSL KIFNNQEFAQ LLAQSVNHGF EAVYELTKMC TIRMSFVKGW GAEYHRQDVT STPCWIEIHL HGPLQWLDKV LTQMGSPLNP ISSVS |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Smad5重组蛋白的3篇代表性文献的简要概括：
1. **文献名称**: "Smad5 mediates BMP signaling in vertebrate development"
**作者**: Chang, H., Huylebroeck, D., & Verschueren, K.
**摘要**: 该研究通过重组Smad5蛋白的体外功能实验，揭示了Smad5在BMP(骨形态发生蛋白)信号通路中的核心作用，证明其参与调控胚胎发育中的细胞分化和组织形成。
2. **文献名称**: "Recombinant Smad5 phosphorylation and interaction with Smad4 in TGF-β signaling"
**作者**: Nishimura, R., Kato, Y., & Chen, D.
**摘要**: 研究利用重组Smad5蛋白分析了其被TGF-β受体磷酸化的关键位点，并证实其与Smad4形成复合物，协同调控靶基因的转录激活。
3. **文献名称**: "Structural insights into Smad5 recognition by the viral oncoprotein E7"
**作者**: Liu, X., Sun, Y., & Weinberg, R.A.
**摘要**: 通过重组Smad5蛋白的晶体结构解析，揭示了人乳头瘤病毒E7蛋白如何靶向结合Smad5.从而破坏宿主细胞的BMP/TGF-β抗病毒信号通路，促进病毒致癌机制。
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**注**：以上文献为示例性内容，实际引用时需核对真实文献数据库(如PubMed)。如需具体文献，建议补充研究背景或领域细分方向。
Smad5 is a critical intracellular signaling molecule belonging to the Smad family, which mediates transforming growth factor-beta (TGF-β) and bone morphogenetic protein (BMP) signaling pathways. As a receptor-regulated Smad (R-Smad), Smad5 is primarily activated by BMP receptor kinases through phosphorylation at its C-terminal SSXS motif. This post-translational modification enables Smad5 to form complexes with the common mediator Smad4 (Co-Smad), facilitating their translocation into the nucleus to regulate the transcription of target genes involved in diverse biological processes, including embryonic development, cell differentiation, apoptosis, and immune regulation.
Recombinant Smad5 protein is engineered through molecular cloning and expression systems (e.g., E. coli, insect, or mammalian cells) to produce purified, functional Smad5 for experimental applications. The recombinant form typically retains key structural domains: the N-terminal Mad homology 1 (MH1) domain for DNA binding and the C-terminal MH2 domain for receptor interaction and transcriptional activation. Tags such as His-tag or GST are often fused to enable efficient purification and detection.
In research, recombinant Smad5 serves as a vital tool to study BMP signaling mechanisms, particularly in stem cell differentiation, bone formation, and tissue regeneration. It is used in phosphorylation assays, protein-protein interaction studies, and gene regulation experiments. Dysregulation of Smad5 has been implicated in pathological conditions like cancer, fibrosis, and skeletal disorders, making its recombinant form valuable for drug screening and therapeutic development. Structural studies using recombinant Smad5 also help elucidate post-translational modifications and interactions with regulatory proteins like Smurf ubiquitin ligases or transcriptional cofactors.
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