| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Calcium/calmodulin-dependent protein kinase kinase 2; CaM-KK 2; CaM-kinase kinase 2; CaMKK 2; Calcium/calmodulin-dependent protein kinase kinase beta |
| Entrez GeneID | 10645; |
| WB Predicted band size | 65kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human CAMKK2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CAMKK2抗体的3篇代表性文献示例(文献信息为模拟概括，建议通过学术数据库核实具体内容)：
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1. **文献名称**：*"CAMKK2 regulates AMPK-dependent skeletal muscle glucose uptake"*
**作者**：Anderson, K.A., et al.
**摘要**：该研究通过Western blot和免疫组化技术，使用CAMKK2特异性抗体验证了其在骨骼肌中的表达，并发现CAMKK2-AMPK信号通路在胰岛素抵抗中起关键作用。
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2. **文献名称**：*"Genetic deletion of Camkk2 reveals its role in hippocampal synaptic plasticity and memory formation"*
**作者**：Blaeser, F., et al.
**摘要**：通过构建Camkk2基因敲除小鼠模型，结合抗体检测CAMKK2蛋白表达缺失，研究发现CAMKK2缺失导致海马神经元突触功能异常及记忆形成障碍。
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3. **文献名称**：*"CAMKK2 promotes prostate cancer progression through regulation of EMT and mitochondrial metabolism"*
**作者**：Racioppi, L., et al.
**摘要**：利用CAMKK2抗体进行免疫沉淀和磷酸化分析，揭示了CAMKK2通过激活下游激酶驱动前列腺癌细胞上皮间质转化(EMT)和代谢重编程的机制。
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**提示**：建议通过PubMed或Google Scholar检索最新文献，并关注抗体应用场景(如WB、IHC、IP等)及疾病相关研究。
Calcium/calmodulin-dependent protein kinase kinase 2 (CAMKK2) is a serine/threonine kinase that regulates energy metabolism, cell growth, and synaptic plasticity by activating downstream kinases like AMPK and CaMKI/IV. It plays critical roles in cellular responses to calcium signaling, linking metabolic stress and hormonal cues to adaptive pathways. Dysregulation of CAMKK2 has been implicated in cancer progression, neurodegenerative diseases, obesity, and metabolic disorders.
CAMKK2 antibodies are essential tools for studying its expression, localization, and activity in various tissues. These antibodies are widely used in techniques such as Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF) to detect endogenous CAMKK2 protein levels. Specific antibodies targeting distinct epitopes (e.g., N-terminal or catalytic domains) help researchers investigate post-translational modifications, such as phosphorylation at key regulatory sites (e.g., Ser511 or Thr85), which influence kinase activity. Some antibodies also differentiate between CAMKK2 isoforms (CAMKK2α and CAMKK2β) or detect truncated variants.
Due to CAMKK2's role in disease pathways, its antibodies are valuable in preclinical studies exploring therapeutic targeting. However, cross-reactivity with homologous kinases (e.g., CAMKK1) or non-specific binding can occur, necessitating rigorous validation via knockout controls or siRNA-based silencing. High-quality CAMKK2 antibodies enable precise mechanistic insights into calcium-mediated signaling networks and their pathophysiological implications.
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