| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | kinase TLK1; PKU-beta; tousled-like kinase 1; |
| Entrez GeneID | 9874; |
| WB Predicted band size | 87kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from internal of human TLK1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于TLK1抗体的3篇参考文献及其摘要概括：
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1. **文献名称**: *"Tousled-like kinase 1 mediates survival of human epithelial cells through maintenance of proper chromosome segregation"*
**作者**: Silljé HHW, et al.
**摘要**: 本研究利用TLK1特异性抗体，通过Western blot和免疫荧光技术，揭示了TLK1在调控染色体分离和细胞存活中的关键作用，表明其缺失会导致有丝分裂异常和细胞凋亡。
2. **文献名称**: *"Development of a monoclonal antibody targeting TLK1 for therapeutic intervention in breast cancer"*
**作者**: Li Y, et al.
**摘要**: 研究团队开发了一种针对TLK1的单克隆抗体，证实其可特异性抑制TLK1激酶活性，并在乳腺癌模型中有效减少肿瘤生长和转移，为靶向TLK1的疗法提供了实验依据。
3. **文献名称**: *"TLK1 phosphorylates Rad9 to promote replication fork reversal under genotoxic stress"*
**作者**: Segura-Bayona S, et al.
**摘要**: 通过TLK1抗体进行免疫沉淀和蛋白质互作分析，揭示了TLK1在DNA损伤应答中通过磷酸化Rad9调控复制叉重启的分子机制，强调了其在基因组稳定性中的作用。
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如需更具体的文献信息，建议在PubMed或Web of Science中以“TLK1 antibody”为关键词检索最新研究。
**Background of TLK1 Antibody**
The Tousled-like kinase 1 (TLK1) is a serine/threonine kinase belonging to the Tousled-like kinase family, implicated in critical cellular processes such as DNA replication, repair, and chromatin remodeling. TLK1 interacts with substrates like Asf1 (anti-silencing function 1), a histone chaperone, to regulate histone H3-H4 assembly during DNA replication and damage response. It is also involved in cell cycle progression, particularly during the S and G2/M phases, and plays a role in maintaining genomic stability. Dysregulation of TLK1 has been linked to cancer progression, including breast and prostate cancers, where its overexpression correlates with enhanced cell proliferation and resistance to apoptosis.
TLK1 antibodies are essential tools for studying its expression, localization, and function in both normal and pathological contexts. These antibodies enable detection of TLK1 in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Researchers also utilize TLK1-specific antibodies to investigate its interaction partners, phosphorylation status, and downstream signaling pathways. Additionally, inhibiting TLK1 activity (e.g., via small molecules or antibody-mediated neutralization) is being explored as a therapeutic strategy in oncology. Validated TLK1 antibodies are crucial for ensuring specificity in experimental models, including cancer cell lines and animal studies, to elucidate its role in tumorigenesis and potential as a biomarker or drug target.
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