| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CP2A7; CPA7; CPAD; CYP2A; CYP2A7 |
| Entrez GeneID | 1549; |
| WB Predicted band size | 56kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from Internal of human CYP2A7. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CYP2A7抗体的3篇参考文献示例(内容基于假设性文献，实际文献可能需要进一步检索验证)：
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1. **文献名称**: "Selective detection of CYP2A7 in human liver microsomes using a novel monoclonal antibody"
**作者**: Hoffman, S.M. et al.
**摘要**: 本研究开发了一种针对CYP2A7的特异性单克隆抗体，通过免疫印迹和免疫组化验证其与CYP2A6等其他同工酶的交叉反应性较低。结果显示CYP2A7在成人肝脏中表达水平极低，可能与假基因特性相关。
2. **文献名称**: "Antibody-based profiling of CYP2A subfamily enzymes in extrahepatic tissues"
**作者**: Raunio, H. et al.
**摘要**: 文章利用多克隆抗体检测CYP2A亚家族(包括CYP2A6、CYP2A7和CYP2A13)在肺和鼻腔组织的表达。研究发现CYP2A7抗体在某些样本中检测到微弱信号，提示其可能存在组织特异性低水平表达。
3. **文献名称**: "Characterization of CYP2A7 genetic variants and protein detection challenges"
**作者**: Zhang, X. et al.
**摘要**: 研究探讨了CYP2A7基因多态性对其蛋白表达的影响，通过商业化抗体进行Western blot分析。结果显示部分变异体因翻译提前终止而无法被抗体识别，提示抗体检测结果需结合基因型分析。
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**注意**：CYP2A7因被归类为假基因，相关抗体研究较少，多数文献集中于其同源酶CYP2A6.建议结合“CYP2A抗体交叉反应”或“假基因蛋白检测”等关键词扩展检索。实际文献需通过PubMed/Google Scholar验证。
The CYP2A7 antibody is a tool used to study the CYP2A7 protein, a member of the cytochrome P450 superfamily involved in drug metabolism and xenobiotic detoxification. CYP2A7. primarily expressed in the liver, is classified as a pseudogene due to genetic polymorphisms and structural variations that impair its enzymatic activity compared to its functional homolog CYP2A6. While CYP2A6 is well-characterized for metabolizing nicotine, coumarin, and certain pharmaceuticals, CYP2A7's biological role remains ambiguous, though it may contribute to endogenous substrate processing or regulatory functions.
CYP2A7 antibodies are critical for investigating its expression patterns, tissue localization, and potential interactions. These antibodies are typically developed using immunogenic peptide sequences unique to CYP2A7 to avoid cross-reactivity with CYP2A6. despite their high sequence similarity. Research applications include immunohistochemistry, Western blotting, and ELISA to explore CYP2A7's presence in hepatic or extrahepatic tissues, its regulation under pathological conditions (e.g., liver disease, cancer), or its role in interindividual metabolic variability.
Current studies focus on clarifying whether CYP2A7 retains residual enzymatic activity, influences CYP2A6 expression, or serves as a biomarker for disease. Challenges in antibody validation include distinguishing CYP2A7 from other CYP2A subfamily members and confirming specificity in samples with low expression levels. Despite its enigmatic function, CYP2A7 remains a subject of interest for understanding genetic diversity in drug metabolism and disease susceptibility.
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