| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RACK1 |
| Uniprot No | P63244 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-317aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MTEQMTLRGT LKGHNGWVTQ IATTPQFPDM ILSASRDKTI IMWKLTRDET NYGIPQRALR GHSHFVSDVV ISSDGQFALS GSWDGTLRLW DLTTGTTTRR FVGHTKDVLS VAFSSDNRQI VSGSRDKTIK LWNTLGVCKY TVQDESHSEW VSCVRFSPNS SNPIIVSCGW DKLVKVWNLA NCKLKTNHIG HTGYLNTVTV SPDGSLCASG GKDGQAMLWD LNEGKHLYTL DGGDIINALC FSPNRYWLCA ATGPSIKIWD LEGKIIVDEL KQEVISTSSK AEPPQCTSLA WSADGQTLFA GYTDNLVRVW QVTIGTR |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于RACK1重组蛋白的经典文献摘要概括：
1. **文献名称**：*RACK1. a receptor for activated C kinase and a homolog of the β subunit of G proteins, inhibits activity of src tyrosine kinases and growth of NIH 3T3 cells*
**作者**：Ron D, Chen CH, et al.
**摘要**：该研究通过重组RACK1蛋白揭示了其作为激活型蛋白激酶C(PKC)的支架蛋白功能，并证明其通过抑制Src酪氨酸激酶活性调控细胞增殖，为RACK1在信号转导和肿瘤抑制中的作用提供了机制证据。
2. **文献名称**：*Structural basis for the interaction of the scaffolding protein RACK1 with protein kinase C*
**作者**：Adams DR, et al.
**摘要**：通过X射线晶体学解析重组RACK1蛋白的三维结构，明确了其与PKC结合的WD40结构域特征，揭示了RACK1通过构象变化调控PKC活性的分子机制。
3. **文献名称**：*RACK1 interacts with hepatitis C virus RNA polymerase (NS5A) and regulates viral replication*
**作者**：Zhao LJ, et al.
**摘要**：利用重组RACK1蛋白进行体外结合实验，发现其直接与丙型肝炎病毒NS5A蛋白相互作用，并通过调控病毒RNA聚合酶活性抑制病毒复制，提示RACK1在宿主抗病毒反应中的潜在作用。
4. **文献名称**：*RACK1 promotes breast carcinoma migration/metastasis and angiogenesis via activating Src-mediated signaling*
**作者**：Li JJ, et al.
**摘要**：通过重组RACK1蛋白过表达实验，证实其通过激活Src激酶信号通路促进乳腺癌细胞迁移和血管生成，为靶向RACK1的癌症治疗策略提供了理论依据。
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以上文献均涉及重组RACK1蛋白在信号转导、结构生物学或疾病机制中的关键作用，可通过PubMed或Web of Science进一步检索获取全文。
**Background of RACK1 Recombinant Protein**
RACK1 (Receptor for Activated C Kinase 1) is a highly conserved scaffold protein belonging to the WD40 repeat protein family, characterized by its seven-bladed β-propeller structure. It was initially identified as an anchoring protein for activated Protein Kinase C (PKC), facilitating its localization and interaction with downstream substrates. Beyond PKC signaling, RACK1 serves as a versatile adaptor molecule, integrating multiple signaling pathways, including those involving tyrosine kinases, G-protein-coupled receptors (GPCRs), and integrins. Its ability to interact with diverse partners enables roles in cell proliferation, migration, apoptosis, and immune responses.
Structurally, RACK1 contains seven WD40 repeats that mediate protein-protein interactions. These repeats form a rigid platform for binding phosphorylated residues or specific motifs on target proteins. RACK1 is ubiquitously expressed across tissues and is implicated in pathological conditions such as cancer, neurodegenerative diseases, and viral infections, where dysregulation of its interactions contributes to disease progression.
Recombinant RACK1 protein is produced via heterologous expression systems (e.g., *E. coli* or insect cells*) to study its biochemical and functional properties. Purified recombinant RACK1 retains the ability to bind PKC and other partners, making it a valuable tool for *in vitro* assays, structural studies, and screening for therapeutic agents targeting RACK1-mediated pathways. Its recombinant form is also used to investigate post-translational modifications (e.g., phosphorylation) that regulate its activity.
Research on RACK1 recombinant protein has advanced understanding of its dual roles as a signaling hub and disease modulator, highlighting its potential as a therapeutic target or diagnostic marker in multiple disorders.
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