| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | KMLS; KRP; MLCK; MYLK; myosin light chain kinase |
| Entrez GeneID | 4638; |
| WB Predicted band size | 200kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human MYLK. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与MYLK抗体相关的研究文献摘要，供参考：
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1. **标题**：*"Myosin light chain kinase regulates endothelial cell barrier function in acute lung injury"*
**作者**：Dudek, S.M., et al.
**摘要**：研究探讨了MYLK在炎症诱导的内皮屏障功能障碍中的作用。通过使用MYLK抗体检测其表达和活性，发现MYLK磷酸化水平升高与肺微血管通透性增加相关，提示其在急性肺损伤中的关键调控机制。
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2. **标题**：*"Tumor-specific expression of myosin light chain kinase isoforms promotes cancer progression"*
**作者**：Zhou, Y., et al.
**摘要**：该研究分析了MYLK不同剪接变体在结肠癌中的表达差异。通过免疫组化(使用MYLK抗体)和基因沉默实验，证实MYLK过表达促进肿瘤细胞侵袭和转移，并与患者预后不良相关。
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3. **标题**：*"Genetic variants in MYLK associated with thoracic aortic dissection"*
**作者**：Shao, L., et al.
**摘要**：研究发现MYLK基因突变与主动脉夹层发病风险相关。利用MYLK抗体检测患者血管组织中蛋白表达，发现突变导致MYLK活性异常，破坏平滑肌收缩功能，最终引发血管壁结构损伤。
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**应用场景**：
这些文献展示了MYLK抗体在疾病机制研究中的广泛应用，包括癌症转移(如结肠癌)、血管疾病(如主动脉夹层)及急性肺损伤模型。研究多通过Western blot、免疫组化或功能性实验验证MYLK的表达及活性，为靶向治疗提供依据。
**Background of MYLK Antibodies**
MYLK (myosin light-chain kinase) is a calcium/calmodulin-dependent enzyme critical for regulating smooth muscle contraction by phosphorylating myosin regulatory light chains. It exists in multiple isoforms, including long (MYLK1) and short (SMLLK) forms, with tissue-specific expression in vascular smooth muscle, endothelial cells, and non-muscle cells. MYLK modulates vascular tone, endothelial barrier function, and inflammatory responses, implicating it in diseases like hypertension, asthma, acute lung injury, and cancer metastasis.
MYLK antibodies are tools used to detect and quantify MYLK protein levels in research. They aid in studying MYLK's role in cellular signaling, cytoskeletal dynamics, and disease mechanisms via techniques like Western blotting, immunohistochemistry, or immunofluorescence. In cancer, MYLK overexpression is linked to tumor progression and metastasis, making these antibodies valuable in oncology research. Conversely, MYLK inhibition in vascular disorders may reduce pathological contraction or leakage.
Commercial MYLK antibodies are typically raised against specific epitopes of human or murine MYLK, with validation in relevant models. Challenges include distinguishing isoforms and ensuring specificity due to structural similarities among kinase family members. Ongoing research focuses on MYLK's therapeutic targeting and its potential as a biomarker, highlighting the antibody's importance in both mechanistic and translational studies.
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