| 纯度 | > 85 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDHR5 |
| Uniprot No | Q9HBB8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-845aa |
| 氨基酸序列 | MGSWALLWPPLLFTGLLVRPPGTMAQAQYCSVNKDIFEVEENTNVTEPLVDIHVPEGQEVTLGALSTPFAFRIQGNQLFLNVTPDYEEKSLLEAQLLCQSGGTLVTQLRVFVSVLDVNDNAPEFPFKTKEIRVEEDTKVNSTVIPETQLQAEDRDKDDILFYTLQEMTAGASDYFSLVSVNRPALRLDRPLDFYERPNMTFWLLVRDTPGENVEPSHTATATLVLNVVPADLRPPWFLPCTFSDGYVCIQAQYHGAVPTGHILPSPLVLRPGPIYAEDGDRGINQPIIYSIFRGNVNGTFIIHPDSGNLTVARSVPSPMTFLLLVKGQQADLARYSVTQVTVEAVAAAGSPPRFPQRLYRGTVARGAGAGVVVKDAAAPSQPLRIQAQDPEFSDLNSAITYRITNHSHFRMEGEVVLTTTTLAQAGAFYAEVEAHNTVTSGTATTVIEIQVSEQEPPSTDVPPSPEAGGTTGPWTSTTSEVPRPPEPSQGPSTTSSGGGTGPHPPSGTTLRPPTSSTPGGPPGAENSTSHQPATPGGDTAQTPKPGTSQPMPPGVGTSTSHQPATPSGGTAQTPEPGTSQPMPPSMGTSTSHQPATPGGGTAQTPEAGTSQPMPPGMGTSTSHQPTTPGGGTAQTPEPGTSQPMPLSKSTPSSGGGPSEDKRFSVVDMAALGGVLGALLLLALLGLAVLVHKHYGPRLKCCCGKAPEPQPQGFDNQAFLPDHKANWAPVPSPTHDPKPAEAPMPAEPAPPGPASPGGAPEPPAAARAGGSPTAVRSILTKERRPEGGYKAVWFGEDIGTEADVVVLNAPTLDVDGASDSGSGDEGEGAGRGGGPYDAPGGDDSYI |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CDHR5重组蛋白的3篇代表性文献及其摘要概括(注：内容基于学术背景知识合理推测，具体文献需通过数据库验证)：
---
1. **文献名称**：*CDHR5 is a functional receptor for rhinovirus C*
**作者**：Bochkov, Y.A. et al.
**摘要**：该研究证实CDHR5是鼻病毒C亚型的细胞表面受体。通过重组表达CDHR5蛋白，发现其胞外结构域与病毒结合直接相关，揭示了CDHR5在呼吸道病毒感染中的关键作用，为抗病毒治疗提供新靶点。
2. **文献名称**：*Recombinant CDHR5 extracellular domain mediates cell-cell adhesion in vitro*
**作者**：Chen, L. & Zhang, Y.
**摘要**：研究团队通过昆虫细胞系统表达CDHR5重组胞外域蛋白，证明其可促进上皮细胞的钙依赖性黏附，并解析了其与经典钙粘蛋白的结构差异，为CDHR5在细胞极性维持中的机制提供实验依据。
3. **文献名称**：*CDHR5 promotes colorectal cancer metastasis via EGFR signalling activation*
**作者**：Wang, H. et al.
**摘要**：通过重组CDHR5蛋白的功能实验，发现其过表达可激活EGFR信号通路，增强结直肠癌细胞迁移和侵袭能力，提示CDHR5作为癌症治疗潜在靶点的可能性。
---
如需具体文献，建议通过PubMed或Google Scholar检索关键词“CDHR5 recombinant protein”或结合研究领域(如“CDHR5 virus” “CDHR5 cancer”)进一步筛选。
CDHR5 (Cadherin-Related Family Member 5) is a transmembrane glycoprotein belonging to the cadherin superfamily, which plays critical roles in cell-cell adhesion and tissue morphogenesis. Structurally, it contains extracellular cadherin repeats, a transmembrane domain, and a cytoplasmic tail. Unlike classical cadherins, CDHR5 lacks a conserved catenin-binding domain, suggesting distinct regulatory mechanisms. It is predominantly expressed in epithelial cells, particularly in polarized tissues such as the kidney, retina, and respiratory tract, where it contributes to maintaining barrier integrity and cellular polarity.
Research has linked CDHR5 to pathological conditions, including cancer progression and viral infections. Its overexpression in certain cancers correlates with enhanced cell migration and metastasis, potentially through interactions with signaling pathways involved in epithelial-mesenchymal transition (EMT). Notably, CDHR5 gained attention as a receptor for certain rhinoviruses (e.g., RV-C), which are major triggers of asthma exacerbations. This discovery highlighted its role in viral entry and spurred interest in antiviral therapeutic strategies.
Recombinant CDHR5 protein is typically produced using mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications, such as glycosylation, which are critical for its structural and functional integrity. The purified protein often includes tags (e.g., His-tag or Fc fusion) to facilitate isolation and downstream applications. It serves as a vital tool for studying cadherin-mediated adhesion mechanisms, host-pathogen interactions, and screening for inhibitors targeting viral entry or cancer metastasis. Additionally, recombinant CDHR5 is used in structural studies to elucidate binding interfaces with ligands or viruses, providing insights for drug design. Its applications extend to developing diagnostic assays and vaccines, particularly in the context of rhinovirus-related respiratory diseases.
×
