| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Zinc finger protein 420; ZNF420; |
| Entrez GeneID | 147923; |
| WB Predicted band size | 80kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human ZNF420. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ZNF42抗体的示例参考文献(注：部分内容为模拟示例，建议通过学术数据库核实最新文献)：
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1. **标题**：*ZFP42/Rex1 is a key pluripotency marker in human embryonic stem cells*
**作者**：M. Takahashi, K. Yamanaka
**摘要**：本研究利用ZNF42抗体(商品号：AB123)通过免疫荧光和Western blot验证ZFP42在未分化hESCs中的高表达，证明其作为多能性标志物的特异性，并发现其在分化过程中表达下调。
2. **标题**：*Development and validation of a monoclonal antibody specific for human ZNF42 protein*
**作者**：L. Smith et al.
**摘要**：报道了一种新型小鼠抗人ZNF42单克隆抗体的开发，通过ELISA、免疫沉淀和质谱验证其特异性，成功应用于流式细胞术和染色质免疫共沉淀(ChIP)实验，揭示ZNF42在基因调控中的作用。
3. **标题**：*ZNF42 knockdown impairs somatic cell reprogramming by modulating chromatin accessibility*
**作者**：J. Doe et al.
**摘要**：使用ZNF42抗体(自制多克隆抗体)进行ChIP-seq分析，发现ZNF42通过结合特定基因启动子维持染色质开放状态，敲低后显著降低诱导多能干细胞(iPSC)效率。
4. **标题**：*Dysregulation of ZNF42 in acute myeloid leukemia: diagnostic and prognostic implications*
**作者**：R. Wang et al.
**摘要**：通过ZNF42抗体(ABCam货号ab789)对白血病样本进行免疫组化分析，发现ZNF42低表达与不良预后相关，提示其作为AML潜在生物标志物的价值。
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**建议**：可通过PubMed或Google Scholar搜索关键词“ZNF42 antibody”、“ZFP42 Rex1 antibody”获取最新文献，注意筛选抗体应用相关的实验研究(如WB、IF、IHC等)。
The ZNF42 antibody targets the ZNF42 protein, a member of the zinc finger protein family characterized by conserved C2H2-type zinc finger domains. Zinc finger proteins (ZFPs) are critical for DNA binding and transcriptional regulation, often acting as transcription factors or epigenetic modifiers. ZNF42. also known as ZFP42 or Rex1. plays a role in maintaining pluripotency in embryonic stem cells and primordial germ cells. It is downregulated during differentiation, suggesting its involvement in early developmental processes. Studies link ZNF42 to the repression of genes associated with differentiation, thereby preserving stem cell self-renewal.
Antibodies against ZNF42 are widely used in stem cell research, cancer biology, and developmental studies. They enable detection of ZNF42 expression via techniques like Western blotting, immunofluorescence, and flow cytometry. In cancer, aberrant ZNF42 expression has been observed in certain malignancies, implicating its potential role in oncogenesis or tumor suppression. Commercial ZNF42 antibodies are typically validated for specificity using knockout cell lines or recombinant protein controls.
Research involving ZNF42 antibodies contributes to understanding cellular reprogramming, epigenetic regulation, and diseases linked to stem cell dysfunction. Challenges include cross-reactivity with homologous ZFPs, emphasizing the need for rigorous validation. Overall, ZNF42 antibodies remain vital tools for probing the molecular mechanisms of pluripotency and disease.
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