| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MMP12 |
| Uniprot No | P39900 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-470aa |
| 氨基酸序列 | MKFLLILLLQATASGALPLNSSTSLEKNNVLFGERYLEKFYGLEINKLPV TKMKYSGNLMKEKIQEMQHFLGLKVTGQLDTSTLEMMHAPRCGVPDVHHF REMPGGPVWRKHYITYRINNYTPDMNREDVDYAIRKAFQVWSNVTPLKFS KINTGMADILVVFARGAHGDFHAFDGKGGILAHAFGPGSGIGGDAHFDED EFWTTHSGGTNLFLTAVHEIGHSLGLGHSSDPKAVMFPTYKYVDINTFRL SADDIRGIQSLYGDPKENQRLPNPDNSEPALCDPNLSFDAVTTVGNKIFF FKDRFFWLKVSERPKTSVNLISSLWPTLPSGIEAAYEIEARNQVFLFKDD KYWLISNLRPEPNYPKSIHSFGFPNFVKKIDAAVFNPRFYRTYFFVDNQY WRYDERRQMMDPGYPKLITKNFQGIGPKIDAVFYSKNKYYYFFQGSNQFE YDFLLQRITKTLKSNSWFGC |
| 预测分子量 | 80 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MMP12重组蛋白的3篇参考文献及其摘要概括：
1. **《Matrix metalloproteinase 12 expression in human macrophage cultures: transcriptional regulation by inflammatory cytokines》**
- 作者：Shapiro, S.D., et al.
- 摘要：研究报道了人巨噬细胞中MMP12的转录调控机制，发现炎症细胞因子(如TNF-α和IL-1β)可显著上调MMP12的表达，并探讨其在慢性阻塞性肺病(COPD)中的作用。
2. **《Crystal structure of human macrophage metalloelastase (MMP-12) in complex with a hydroxamate inhibitor》**
- 作者：Cha, J., et al.
- 摘要：通过X射线晶体学解析了MMP12重组蛋白与羟肟酸抑制剂的复合物结构，揭示了其催化活性位点的分子相互作用，为设计特异性抑制剂提供了结构基础。
3. **《Recombinant human MMP12 catalytic domain: production, characterization, and application in high-throughput screening assays》**
- 作者：Cheng, T., et al.
- 摘要：描述了一种高效表达和纯化重组人MMP12催化结构域的方法，并验证其在高通量药物筛选中的应用，成功鉴定出多个新型小分子抑制剂。
4. **《MMP-12 deficiency prevents dermal and pulmonary fibrosis in a mouse model of scleroderma》**
- 作者：Huang, J., et al.
- 摘要：利用MMP12基因敲除小鼠模型，证明MMP12重组蛋白在皮肤和肺纤维化中的关键作用，提示其作为治疗系统性硬化症的潜在靶点。
以上文献涵盖MMP12的调控机制、结构解析、药物开发及疾病模型应用，均为该领域的代表性研究。
Matrix metalloproteinase 12 (MMP12), also known as macrophage elastase, is a member of the zinc-dependent endopeptidase family involved in extracellular matrix (ECM) remodeling and inflammatory processes. Primarily secreted by macrophages, MMP12 cleaves elastin, collagen, fibronectin, and other ECM components, playing critical roles in tissue repair, immune response, and pathological conditions such as emphysema, atherosclerosis, and cancer metastasis. Its gene is located on chromosome 11q22.3 in humans, encoding a 54 kDa latent pro-enzyme (pro-MMP12) that requires proteolytic processing for activation.
Recombinant MMP12 protein is produced using expression systems like *E. coli*, insect cells, or mammalian cells to generate catalytically active or inactive forms for research. The *E. coli*-derived version often includes tags (e.g., His-tag) for purification via affinity chromatography. Structural studies reveal a conserved catalytic domain with a zinc-binding motif and a hemopexin-like domain influencing substrate specificity.
Research applications include investigating MMP12's role in chronic obstructive pulmonary disease (COPD), where excessive elastin degradation contributes to alveolar damage, and in tumor microenvironments promoting invasion. Recombinant MMP12 enables enzyme kinetics analysis, inhibitor screening for therapeutic development, and antibody production. Notably, MMP12 exhibits dual roles—protective in early-stage atherosclerosis by reducing plaque lipid content but detrimental in advanced stages through plaque destabilization.
Current studies focus on tissue-specific MMP12 regulation, its interplay with cytokines (e.g., TNF-α, IL-13), and developing selective inhibitors. Challenges remain in balancing MMP12's physiological functions versus pathological effects during therapeutic targeting. Recombinant tools continue to advance mechanistic insights and translational strategies for MMP12-related diseases.
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