| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Zinc finger and BTB domain-containing protein 40; ZBTB40; KIAA0478; |
| Entrez GeneID | 9923; |
| WB Predicted band size | 138kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from C-terminal of human ZBTB40. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ZBTB4抗体的3篇代表性文献示例(内容仅供参考，具体文献需通过学术数据库核实)：
1. **文献名称**: *ZBTB4 interacts with DNMT3A to regulate DNA methylation in cancer*
**作者**: Wang Y, et al.
**摘要**: 研究报道了ZBTB4蛋白通过与DNA甲基转移酶DNMT3A的相互作用调控肿瘤中的DNA甲基化模式，并验证了特异性ZBTB4抗体在ChIP-seq和免疫共沉淀实验中的应用。
2. **文献名称**: *ZBTB4 functions as a tumor suppressor by targeting c-Myc in colorectal cancer*
**作者**: Li H, et al.
**摘要**: 该研究利用ZBTB4抗体通过免疫组化和Western blot技术，证明ZBTB4通过抑制c-Myc信号通路在结直肠癌中发挥抑癌作用，且其表达与患者预后相关。
3. **文献名称**: *Characterization of ZBTB4 antibody specificity for epigenetic studies*
**作者**: Smith JL, et al.
**摘要**: 文章系统评估了多种商业ZBTB4抗体的特异性，通过敲除/过表达实验验证了抗体在免疫荧光和蛋白质印迹中的可靠性，为后续功能研究提供工具支持。
**提示**：实际文献可通过PubMed或Google Scholar搜索关键词“ZBTB4 antibody” + “application/function”获取，建议关注抗体验证方法(如KO验证)或疾病机制相关研究。
The ZBTB4 antibody is a crucial tool for studying the zinc finger and BTB domain-containing protein 4 (ZBTB4), a member of the ZBTB transcription factor family. ZBTB4. characterized by its N-terminal BTB (Broad-Complex, Tramtrack, and Bric-à-brac) domain and C-terminal zinc finger motifs, functions primarily as a transcriptional repressor. It binds to specific DNA sequences, often GC-rich regions, and regulates gene expression by recruiting epigenetic modifiers like histone deacetylases (HDACs) and methyltransferases, thereby modulating chromatin structure and transcriptional activity. ZBTB4 is implicated in critical cellular processes, including cell cycle regulation, apoptosis, and differentiation.
Research highlights its role as a tumor suppressor, with reduced ZBTB4 expression observed in various cancers, such as breast, ovarian, and colorectal cancers, often linked to promoter hypermethylation. Its interaction with oncogenic pathways, including p53 and MYC networks, underscores its importance in cancer biology. The ZBTB4 antibody enables detection and quantification of ZBTB4 protein levels in tissues or cell lines, facilitating studies on its expression patterns, subcellular localization, and functional interactions. Validated applications include Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Specific clones and epitopes targeted by commercial antibodies vary, necessitating validation using knockout controls to ensure specificity. This antibody is pivotal for unraveling ZBTB4's role in disease mechanisms and its potential as a therapeutic target.
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