| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | dkfzp451j1719; duba8; otu1; otud2; pro0907 |
| Entrez GeneID | 55432; |
| WB Predicted band size | 35kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | Synthesized peptide derived from internal of human YOD1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于YOD1抗体的参考文献及其摘要概括：
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1. **文献名称**：*YOD1/TRAF6 association balances p62-dependent IL-1 signaling to NF-κB*
**作者**：Schaubeck, S. et al.
**摘要**：本研究揭示了YOD1通过与TRAF6相互作用调控IL-1信号通路的机制，利用YOD1特异性抗体进行免疫共沉淀实验，证明YOD1通过去泛素化作用平衡p62依赖的NF-κB激活，影响炎症反应。
2. **文献名称**：*The deubiquitinating enzyme YOD1 regulates the apoptosis/cell proliferation balance in colorectal cancer*
**作者**：Li, Y. et al.
**摘要**：通过Western blot和免疫组化实验(使用YOD1抗体)，作者发现YOD1在结直肠癌中高表达，并通过调控Bax/Bcl-2信号轴影响细胞凋亡与增殖的平衡，提示其作为潜在治疗靶点。
3. **文献名称**：*YOD1 controls the activation threshold of the unfolded protein response by modulating Ire1 stability*
**作者**：Kuo, C.L. et al.
**摘要**：研究利用YOD1敲除细胞系及抗体验证，表明YOD1通过去泛素化调控内质网应激感应蛋白Ire1α的稳定性，影响未折叠蛋白反应(UPR)的激活阈值，参与细胞应激适应性调节。
4. **文献名称**：*Development and characterization of a monoclonal antibody specific for YOD1 deubiquitinase*
**作者**：Hrdinka, M. et al.
**摘要**：本文描述了一种新型YOD1单克隆抗体的开发与验证，通过ELISA、免疫荧光和流式细胞术证明其高特异性，适用于检测内源性YOD1蛋白表达及定位，为后续功能研究提供工具。
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以上文献均涉及YOD1抗体的应用，涵盖机制研究、疾病关联及抗体开发，可作为相关研究的参考。
YOD1. also known as OTUD2. is a deubiquitinating enzyme (DUB) belonging to the ovarian tumor (OTU) domain protease family. It plays a critical role in regulating ubiquitin-dependent cellular processes, including protein quality control, endoplasmic reticulum-associated degradation (ERAD), and cell cycle progression. YOD1 catalyzes the removal of ubiquitin chains from substrate proteins, thereby modulating their stability, localization, or interaction partners. Its activity is closely linked to the ubiquitin-proteasome system (UPS), a key pathway for maintaining proteostasis. Dysregulation of YOD1 has been implicated in various diseases, such as cancer, neurodegenerative disorders, and immune dysfunctions, highlighting its importance in cellular homeostasis.
YOD1 antibodies are essential tools for investigating the expression, localization, and function of YOD1 in biological systems. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunoprecipitation to study YOD1’s involvement in cellular stress responses, apoptosis, and autophagy. Researchers also utilize YOD1 antibodies to explore its interaction with other ERAD components, such as VCP/p97 and Derlin-1. shedding light on mechanistic pathways. Commercially available YOD1 antibodies vary in specificity and host species, requiring validation for experimental contexts. Understanding YOD1’s regulatory mechanisms through antibody-based assays contributes to therapeutic strategies targeting ubiquitination pathways in disease.
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