关键信息
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基因名
xylF
- 应用
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别名
xylF；xylT；D-xylose-binding periplasmic Protein
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P37387
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表达区间
24-330aa
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氨基酸序列
KEVKIGM AIDDLRLERW QKDRDIFVKK AESLGAKVFV QSANGNEETQ MSQIENMINR GVDVLVIIPY NGQVLSNVVK EAKQEGIKVL AYDRMINDAD IDFYISFDNE KVGELQAKAL VDIVPQGNYF LMGGSPVDNN AKLFRAGQMK VLKPYVDSGK IKVVGDQWVD GWLPENALKI MENALTANNN KIDAVVASND ATAGGAIQAL SAQGLSGKVA ISGQDADLAG IKRIAAGTQT MTVYKPITLL ANTAAEIAVE LGNGQEPKAD TTLNNGLKDV PSRLLTPIDV NKNNIKDTVI KDGFHKESEL
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分子量
35.7 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
The study of the xylF gene, which encodes for a key enzyme involved in the degradation of xylenes, is significant in the context of bioremediation and environmental biotechnology. Xylenes, commonly found as pollutants in soil and groundwater due to industrial activities, pose environmental and health risks. The xylF gene is part of the xylene degradation pathway in certain bacteria, such as Pseudomonas species, facilitating the conversion of toxic xylenes into less harmful compounds. Research on the recombinant protein produced by the xylF gene aims to elucidate the enzyme's structure, function, and catalytic mechanisms. By expressing the xylF gene in a suitable host, scientists can obtain large amounts of the enzyme for detailed biochemical studies. Understanding the properties of this recombinant protein not only enhances the fundamental knowledge of microbial metabolism but also informs strategies for developing microbial strains or enzymes that can be applied to efficiently bioremediate contaminated sites, thereby contributing to environmental conservation efforts. Ultimately, the insights gained from xylF protein studies can lead to innovative solutions for managing xylene pollution and improving the efficacy of bioremediation processes.
