| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TSC22D1 |
| Uniprot No | Q15714 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1073aa |
| 氨基酸序列 | MHQPPESTAAAAAAADISARKMAHPAMFPRRGSGSGSASALNAAGTGVGSNATSSEDFPPPSLLQPPPPAASSTSGPQPPPPQSLNLLSQAQLQAQPLAPGGTQMKKKSGFQITSVTPAQISASISSNNSIAEDTESYDDLDESHTEDLSSSEILDVSLSRATDLGEPERSSSEETLNNFQEAETPGAVSPNQPHLPQPHLPHLPQQNVVINGNAHPHHLHHHHQIHHGHHLQHGHHHPSHVAVASASITGGPPSSPVSRKLSTTGSSDSITPVAPTSAVSSSGSPASVMTNMRAPSTTGGIGINSVTGTSTVNNVNITAVGSFNPNVTSSMLGNVNISTSNIPSAAGVSVGPGVTSGVNVNILSGMGNGTISSSAAVSSVPNAAAGMTGGSVSSQQQQPTVNTSRFRVVKLDSSSEPFKKGRWTCTEFYEKENAVPATEGVLINKVVETVKQNPIEVTSERESTSGSSVSSSVSTLSHYTESVGSGEMGAPTVVVQQQQQQQQQQQQQPALQGVTLQQMDFGSTGPQSIPAVSIPQSISQSQISQVQLQSQELSYQQKQGLQPVPLQATMSAATGIQPSPVNVVGVTSALGQQPSISSLAQPQLPYSQAAPPVQTPLPGAPPPQQLQYGQQQPMVSTQMAPGHVKSVTQNPASEYVQQQPILQTAMSSGQPSSAGVGAGTTVIPVAQPQGIQLPVQPTAVPAQPAGASVQPVGQAPAAVSAVPTGSQIANIGQQANIPTAVQQPSTQVPPSVIQQGAPPSSQVVPPAQTGIIHQGVQTSAPSLPQQLVIASQSSLLTVPPQPQGVEPVAQGIVSQQLPAVSSLPSASSISVTSQVSSTGPSGMPSAPTNLVPPQNIAQTPATQNGNLVQSVSQPPLIATNTNLPLAQQIPLSSTQFSAQSLAQAIGSQIEDARRAAEPSLVGLPQTISGDSGGMSAVSDGSSSSLAASASLFPLKVLPLTTPLVDGEDESSSGASVVAIDNKIEQAMDLVKSHLMYAVREEVEVLKEQIKELIEKNSQLEQENNLLKTLASPEQLAQFQAQLQTGSPPATTQPQGTTQPPAQPASQGSGPTA |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TSC22D1重组蛋白的3篇参考文献示例(文献信息为模拟内容，仅供参考)：
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1. **文献名称**: *"Cloning and functional characterization of TSC22D1 as a TGF-β-responsive transcriptional regulator"*
**作者**: Kester HA, et al.
**摘要**: 研究报道了TSC22D1基因的克隆及其重组蛋白的表达，发现其在TGF-β信号通路中作为转录调节因子，调控靶基因表达和细胞凋亡过程。
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2. **文献名称**: *"Recombinant TSC22D1 suppresses renal fibrosis by modulating SMAD signaling in vitro and in vivo"*
**作者**: Hofmann A, et al.
**摘要**: 通过在大鼠肾细胞中表达重组TSC22D1蛋白，证实其通过抑制SMAD蛋白磷酸化减轻纤维化，为肾脏疾病治疗提供潜在靶点。
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3. **文献名称**: *"TSC22D1 recombinant protein interacts with p53 to regulate oxidative stress-induced apoptosis"*
**作者**: Dutta S, Fanburg BL.
**摘要**: 研究利用重组TSC22D1蛋白进行体外结合实验，发现其与p53直接互作，调控氧化应激条件下的细胞凋亡反应。
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*注：以上文献信息为示例性内容，实际文献需通过PubMed、Google Scholar等数据库检索确认。建议结合关键词“TSC22D1 recombinant protein”“TSC22D1 expression”进一步查阅。*
**Background of Recombinant TSC22D1 Protein**
TSC22D1 (TGF-β-stimulated clone 22 domain family member 1) is a conserved protein belonging to the TSC22D family, initially identified as a downstream target of the TGF-β signaling pathway. It encodes a transcription regulator containing a TSC22 domain, which mediates protein-protein and protein-DNA interactions. TSC22D1 is ubiquitously expressed across tissues and localizes to both the nucleus and cytoplasm, suggesting roles in diverse cellular processes, including proliferation, differentiation, apoptosis, and stress responses.
Functionally, TSC22D1 acts as a context-dependent modulator of signaling pathways, such as TGF-β, NF-κB, and glucocorticoid signaling. It has been implicated in regulating gene expression by interacting with transcription factors or chromatin modifiers. Studies link TSC22D1 to pathological conditions, including cancer (as a potential tumor suppressor or promoter, depending on cellular context), fibrosis, and inflammatory diseases. For example, reduced TSC22D1 expression correlates with tumor progression in certain cancers, while its upregulation may contribute to fibrotic tissue remodeling.
Recombinant TSC22D1 protein is typically produced using bacterial (e.g., *E. coli*) or mammalian expression systems to ensure proper folding and post-translational modifications. The purified protein serves as a molecular tool to study its biochemical activity, interactome, and regulatory mechanisms *in vitro*. Researchers utilize it in assays like electrophoretic mobility shift assays (EMSAs), co-immunoprecipitation, or cell-based studies to dissect its role in signaling cascades and disease pathways. Its recombinant form also holds potential for therapeutic exploration, such as targeting fibrosis or inflammation. Understanding TSC22D1's structure-function relationships through recombinant protein studies remains critical for elucidating its dual roles in health and disease.
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