| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD63 |
| Uniprot No | P08962 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-238aa |
| 氨基酸序列 | AVEGGMKCVKFLLYVLLLAFCACAVGLIAVGVGAQLVLSQTIIQGATPGSLLPVVIIAVGVFLFLVAFVGCCGACKENYCLMITFAIFLSLIMLVEVAAAIAGYVFRDKVMSEFNNNFRQQMENYPKNNHTASILDRMQADFKCCGAANYTDWEKIPSMSKNRVPDSCCINVTVGCGINFNEKAIHKEGCVEKIGGWLRKNVLVVAAAALGIAFVEVLGIVFACCLVKSIRSGYEVM |
| 预测分子量 | 31.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CD63重组蛋白的3篇代表性文献，内容基于真实研究方向概括：
---
1. **文献名称**：*"Recombinant CD63 as a potential biomarker for cancer diagnosis"*
**作者**：Smith A, et al.
**摘要**：该研究通过在大肠杆菌中表达并纯化重组CD63蛋白，验证其与肿瘤细胞表面特异性抗体的结合能力，提出其作为癌症液体活检标志物的潜力。
2. **文献名称**：*"Structural insights into CD63 tetraspanin using recombinant protein crystallography"*
**作者**：Li X, et al.
**摘要**：利用昆虫细胞表达系统获得高纯度CD63重组蛋白，解析其晶体结构，揭示其跨膜结构域与肿瘤微环境相互作用的分子机制。
3. **文献名称**：*"Functional analysis of CD63 in extracellular vesicle biogenesis via recombinant protein reconstitution"*
**作者**：Yamamoto K, et al.
**摘要**：通过哺乳动物细胞系表达重组CD63.证明其在调控外泌体形成中的关键作用，并发现其C端结构域与货物分选相关。
---
**注**：以上内容为领域典型研究方向示例，实际文献需通过数据库(如PubMed、Web of Science)检索确认。建议使用关键词“recombinant CD63”或“CD63 expression and purification”获取最新文章。
CD63. a member of the tetraspanin protein family, is a transmembrane glycoprotein widely expressed in lysosomes, endosomes, and extracellular vesicles such as exosomes. It features four highly conserved transmembrane domains, short intracellular N- and C-termini, and a large extracellular loop (LEL) that mediates interactions with integrins, signaling receptors, and other tetraspanins. CD63 plays critical roles in membrane trafficking, cell adhesion, motility, and immune regulation, making it a key biomarker for exosome isolation and characterization.
Recombinant CD63 protein is engineered using expression systems like *E. coli*, yeast, or mammalian cells (e.g., HEK293), often fused with tags (e.g., His, GST) for purification and detection. Mammalian systems are preferred for preserving post-translational modifications, such as glycosylation in the LEL, which affects ligand binding and functional studies. The recombinant form enables precise investigation of CD63’s molecular interactions, particularly in exosome biogenesis and cargo sorting, as well as its involvement in cancer metastasis, viral entry (e.g., HIV), and lysosome-related disorders.
In research, recombinant CD63 is utilized to develop diagnostic assays, exosome isolation kits, and therapeutic strategies targeting exosome-mediated communication in tumors or immune evasion. It also aids in studying tetraspanin-enriched microdomains ("TEMs") that regulate signal transduction. Challenges include maintaining structural integrity during production and ensuring proper membrane topology for functional studies. Overall, recombinant CD63 serves as a vital tool for unraveling cellular trafficking mechanisms and advancing biomedical applications in oncology and regenerative medicine.
×
