| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CASK |
| Uniprot No | O14936-4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-898aa |
| 氨基酸序列 | MADDDVLFEDVYELCEVIGKGPFSVVRRCINRETGQQFAVKIVDVAKFTS SPGLSTEDLKREASICHMLKHPHIVELLETYSSDGMLYMVFEFMDGADLC FEIVKRADAGFVYSEAVASHYMRQILEALRYCHDNNIIHRDVKPHCVLLA SKENSAPVKLGGFGVAIQLGESGLVAGGRVGTPHFMAPEVVKREPYGKPV DVWGCGVILFILLSGCLPFYGTKERLFEGIIKGKYKMNPRQWSHISESAK DLVRRMLMLDPAERITVYEALNHPWLKERDRYAYKIHLPETVEQLRKFNA RRKLKGAVLAAVSSHKFNSFYGDPPEELPDFSEDPTSSGLLAAERAVSQV LDSLEEIHALTDCSEKDLDFLHSVFQDQHLHTLLDLYDKINTKSSPQIRN PPSDAVQRAKEVLEEISCYPENNDAKELKRILTQPHFMALLQTHDVVAHE VYSDEALRVTPPPTSPYLNGDSPESANGDMDMENVTRVRLVQFQKNTDEP MGITLKMNELNHCIVARIMHGGMIHRQGTLHVGDEIREINGISVANQTVE QLQKMLREMRGSITFKIVPSYRTQSSSCEDLPSTTQPKGRQIYVRAQFEY DPAKDDLIPCKEAGIRFRVGDIIQIISKDDHNWWQGKLENSKNGTAGLIP SPELQEWRVACIAMEKTKQEQQASCTWFGKKKKQYKDKYLAKHNADLVTY EEVVKLPAFKRKTLVLLGAHGVGRRHIKNTLITKHPDRFAYPIPHTTRPP KKDEENGKNYYFVSHDQMMQDISNNEYLEYGSHEDAMYGTKLETIRKIHE QGLIAILDVEPQALKVLRTAEFAPFVVFIAAPTITPGLNEDESLQRLQKE SDILQRTYAHYFDLTIINNEIDETIRHLEEAVELVCTAPQWVPVSWVYVD HHHHHH |
| 预测分子量 | 103 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CASK重组蛋白的3篇代表性文献的简要概括(注：文献信息为模拟示例，实际引用需核实)：
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1. **文献名称**：*Structural insights into the Ca²⁺/calmodulin-dependent serine protein kinase CASK*
**作者**：LaConte, L.E.W. et al.
**摘要**：该研究通过重组表达人源CASK蛋白的催化结构域，利用X射线晶体学解析了其三维结构，揭示了其与钙调蛋白结合的分子机制，为理解CASK在神经元信号转导中的作用提供了结构基础。
2. **文献名称**：*Recombinant CASK protein enhances synaptic vesicle clustering in vitro*
**作者**：Mukherjee, K. et al.
**摘要**：作者通过在大肠杆菌中表达并纯化CASK重组蛋白，证明其与突触囊泡蛋白Neurexin的相互作用，并发现CASK可促进体外培养神经元中突触囊泡的聚集，提示其参与突触形成的功能。
3. **文献名称**：*Functional analysis of CASK mutations in neurodevelopmental disorders using recombinant proteins*
**作者**：Hsueh, Y.P. et al.
**摘要**：研究团队构建了多个X连锁智力障碍相关的CASK突变体重组蛋白，通过体外激酶实验和蛋白质结合实验，揭示了突变导致CASK酶活性和支架功能受损的分子机制。
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如需实际文献，建议在PubMed或Google Scholar中检索关键词“CASK recombinant protein”或“CASK purification”，重点关注分子生物学及神经科学领域期刊(如*Journal of Biological Chemistry*、*Neuron*)。
CASK (Calcium/Calmodulin-Associated Serine Kinase) is a multidomain scaffolding protein encoded by the *CASK* gene, belonging to the membrane-associated guanylate kinase (MAGUK) family. It plays a critical role in synaptic organization, neural development, and signal transduction by interacting with proteins like neurexins, syndecans, and transcription factors. Structurally, CASK contains a CaMK-like domain, PDZ domains, SH3 domains, and a guanylate kinase (GuK) domain, enabling diverse molecular interactions. Its involvement in stabilizing synaptic structures, regulating ion channels, and modulating gene expression underscores its importance in brain function.
Recombinant CASK proteins are engineered using expression systems (e.g., *E. coli*, HEK293. or insect cells*) to study CASK’s biochemical properties, interactions, and pathogenetic mechanisms. These purified proteins retain functional domains, allowing researchers to explore CASK’s role in neurodevelopmental disorders like X-linked intellectual disability (XLID), microcephaly, and FG syndrome. Mutations in *CASK* are linked to altered synaptic plasticity and impaired neural connectivity, making recombinant CASK vital for elucidating disease pathways.
Applications include *in vitro* binding assays, structural analysis, drug screening, and functional studies using cell or animal models. Recombinant CASK also aids in developing diagnostic tools and targeted therapies. Despite challenges in maintaining post-translational modifications during production, advances in expression systems continue to enhance protein stability and activity. Overall, CASK recombinant proteins serve as essential tools for advancing neuroscience research and therapeutic innovation.
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